Lypd8 (NM_027339) Mouse Untagged Clone

CAT#: MC201454

Lypd8 (untagged) - Mouse RIKEN cDNA 2210415F13 gene (2210415F13Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lypd8
• Synonyms: 2210415F13Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC026828 sequence for NM_027339
  TTCCATCTTCCTCCGGATAGTGTCCCTGGCTGGACCCAGAAGGTTTGAGGCTCTGTGGCCCCATCCTCTG GGGAAGCCAACTCCAGCATCATGAGAGGCGTCTTCATCGCTGGTGTCATTGCGGCATTCGCCATCACAGT TGTAGACTCCCTGAATTGTACACAGTGTCATACGTACAATAGCACCTGTGATGGCCAGGCCACTGAATGC AATGAGCAGTCCTTCAGTTGTGTGGAGTCCTCTATCAACTCCACTCTAGGAGGGTTCCTGCATGTGTACC AGAACAAGTTCTGCTCCGAATCGAATTGCACCAAGAACAGCACAGAGGTGGCCTTCACTGTCCATCTATT TGATGACCAAAGATACCATTTTGCAAGCCAGTGCTGCCAAGGAGAGTCCTGCAATGCCACCCACTCTGAA TCCGGAACACAGAATGTAACTGACATGCAGTGCATGTCTTGCTATGGTCACAACAAAACACTCTGCGAGG AGAAACCCCAAAAGTGTTACGAAGGAGAACAATGTGTCTTCATCATTGCGGAGATGGTAAATGGCTCTGG CAGAGTGGAGCTGAAGGGCTGTTCTGATATCAGCAACTCTACTTGTCAATTCCTGTCCCCTGGGAACACA ACAGTTGGAGAGTTTGTTTTCAAGTCGGTTGAATGTACGCAACCCACTGAGTATACCAACTCGACCACCA CTATCCCACCAATCACCAACAGTTCCCTCGCCTCTGTTACCCCACAAATCACCAACACTTCCCTCACCTC TGTTACCCGACCAGGCATCAAAACTTCCCCCGCCTCTGTTACCCCACAGGCTAGCATGGGCACCAAAGCT TCCTTCACCTCCTCTATCTTCGGCAGCCTTCTTCTTCTGAAGCTGCTGTTCTGAGGTTCCTGGGCTCTGC TCCATCCAGTATCCCATGGGTGTCAACACCTTTCTCTTCATATACCTTCCAGTTAGACTGTCCAGTGGGT TGGAAGTCACAGGACTCCAGGCAATGCTAGCAGCCACAGTATCTCCTTTATTAAAGTGTTGCTTGAGGTC TAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_027339
• Insert Size: 804 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026828, AAH26828
• RefSeq Size: 1070 bp
• RefSeq ORF: 804 bp
• Locus ID: 70163
• UniProt ID: Q9D7S0
• Cytogenetics: 11 B1.3
• Gene Summary: This gene encodes a member of the Ly6/PLAUR family of cysteine-rich proteins that plays an important role in the protection of colonic epithelium from flagellated microbiota. The encoded protein undergoes proteolytic processing to generate a mature, glycosylphosphatidylinositol-anchored protein that is localized to the apical surface of the colonic epithelial cells. Mice lacking the encoded protein are sensitive to chemically induced intestinal inflammation. [provided by RefSeq, Aug 2016]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 - 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.