Dgkg (NM_138650) Mouse Untagged Clone

CAT#: MC201196

Dgkg (untagged) - Mouse diacylglycerol kinase, gamma (Dgkg), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgkg
• Synonyms: 90kDa; 2900055E17Rik; AI854428; Dagk3; E430001K23Rik; mKIAA4131
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC015278 sequence for NM_138650
  GTGTCTCAAGGCAATCACACTCGGCTGAAAGATGAGCGAAGAACAATGGGTCTCTCTCTCATCAGAAGAA TTTGATCAACTCCAGAAATACTCTGAATATTCCTCCAAAAAGATAAAAGATGTATTGGCTGAGTTTAATG AAGGTGGAAGCCTCAGGCAGTATGACCCACACAAGCCCATTAGCTATGATGTCTTCAAGCTGTTCATGAG GGCATACCTGGAGGTGGACCTTCCTCAGCCACTGAGCACACACCTCTTCCTGGCCTTCAGCCAGAAGCCC AGACAAGAGACACCTGACCATCCAAAGGAGGGGGCCAGCAGTAGTGAGCCCAATGTCTCAGATTATAATT CGGATAATGCTGCCAAAGCAGATGAGGCCTGTGCTCCAGACACTGAATCAAAAACAACTAAGACACAAGC ACCATCTAAAGAACTAGAGGCTGCAGCCCCATGGGAAGACCCAGGTGCTCTGGCTTCAAGCTCAGATGCT CCCGTAGTGTACCTGAAGGATGTCGTGTGTTACTTGTCCCTGATGGAGACCGGGCGACCTCAGGATAAGC TAGAATTCATGTTTCGTCTCTATGATTCGGATGAAAATGGACTCCTGGACCAAGCGGAGATGGATCAGAT TGTGAGCCAAATGCTGCACGTTGCCCAGTACCTGGAATGGGATCCCACAGAGCTCAGACCTATACTGAAG GAGATGCTGCAAGGCATGGACTATGACAAGGATGGCTTCGTGTCTCTACAGGAGTGGATCAACGGGGGAA TGACCACCATCCCTTTGCTGGTCCTCCTGGGAATGGATGACTCTGGCTCCAAGGGCGATGGGAGGCATGC CTGGACCCTGAAGCACTTCAAGAAGCCCACTTACTGCAACTTCTGCCGCGCCATGCTGATGGGTGTTGGG AAGCAAGGCCTGTGCTGCATCTACTGTAAATATACAGTCCACCAACGCTGTGTATCCAAAACCATTCATG GATGTGTGAAAACAAACTCCAAAGCCAAAAGGAGTGGCGAGGTGATGCAGCATGCGTGGGTGGAAGGGAA CTCCTCCGTCAAGTGTGACCGGTGCCACAAAAGTATCAAGTGCTACCAGAGTGTCACCGCGCGGCACTGC GTGTGGTGCCGGATGACGTTTCACCGCAAATGTGAATTATCGACAGTGTGCGACGGTGGGGAACTCAAAG ACCACATTTTGCTGCCTACCTCCATATGCCCCGTCTCCGGAGACCGGCAAGGAGGGAAGTCCGATGGCAG TGTGGCCGCCAAGGGTGAACTTGTAACTCAGTATAAGATCATCCCTAGCCCGGGGACGCATCCCCTGCTG GTCCTGGTGAACCCCAAGAGTGGAGGAAGACAAGGAGAAAGAATTCTTCGGAAATTTCACTACCTGCTCA ACCCCGAACAAGTTTTCAACCTGGACAATGGGGGACCTACACCAGGATTGAACTTCTTTCATGATACCCC AGACTTCCGTGTTCTTGCCTGTGGCGGAGATGGGACCGTTGGCTGGATTTTGGATTGCATTGATAAGGCT AACTTCACAAAGCACCCACCGGTGGCTGTCCTGCCTCTTGGAACAGGGAATGACCTCGCACGATGCCTCC GCTGGGGAGGAGGTTACGAAGGGGGCAGCTTGACCAAAATCCTGAAGGAAATTGAACAGAGCCCCTTGGT GATGCTGGACCGTTGGTACCTAGAGGTCATGCCCAGGGAAGAGGTGGAGAATGGAGACCAAGTCCCATAC AACATCATGAACAACTATTTCTCCATTGGTGTGGATGCTTCAATTGCACACAGATTCCATATGATGAGAG AGAAACACCCTGAAAAATTCAACAGCAGGATGAAGAACAAGCTGTGGTATTTCGAATTTGGTACCTCGGA AACCTTTGCAGCCACCTGCAAGAAACTCCATGACCACATAGAGCTGGAGTGTGACGGAGTTGAGGTGGAT CTGAGCAACATCTTCCTGGAAGGCATCGCCATTCTTAATATTCCCAGCATGTACGGAGGCACCAATCTCT GGGGAGAAACCAAGAAGAACCGGGCTGTGATCCGGGAAAGCAGGAAGAGCGTCACAGACCCAAAGGAGCT GAAATGCTGTGTCCAAGACCTCAGCGATCAGCTCCTTGAAGTGGTGGGTCTAGAGGGAGCCATGGAGATG GGACAGATCTACACTGGCCTGAAGAGTGCGGGCAGGAGACTGGCCCAGTGCTCCTCTGTCACCATTAGGA CTAACAAGCTTCTGCCGATGCAAGTAGACGGAGAGCCCTGGATGCAGCCACAATGCACGATTAAAATCAC TCACAAAAACCAGGCACCTATGATGATGGGGCCTCCCCAGAAGAGCAGCTTTTTTTCTCTGAGGAGGAAA AGCCGTTCAAAAGACTAAACAATATGCCAAACACCAGCTCTACTGAGAAAGGAAAGAAGAATCTACACAC ACACACACACACACACACACACACACACACACACACACCTGTAACCAGAGGAAGCCAAGCCACCTTCCAG AGGAAAGTGCAATCCTGCCATGCAGTCTACCTGTTTTGAAAATGGATCCACTCTTAACAGAGCCAATACC CATGAAATATTCCAAATGGACTCAGGGCCCACGAAGATGGGTTGGCTCATGCAGCAACTTTCACATTCCC ACAGGCTTTGGGTAGACTTTGAGCCTGCAGGAGAAATCTCCCTTTTTCTGTGCCACACTCCTGTAAGGCT CCAGGTTGTAGGTTTCCTGCCTACAGCAAGATCGGATGTGGTCAAGGGAAAGAGCTCCAACCCAGAAACC TTGCACAAAAGTCCTTCTGTATAAAAACAAAACAGCCCACAGCTCTGCAAGCAGAAGCCTTGGCAGGGCT CGGCGAGCACAGGATGGTTGCTTTGCTGTCCTTCAAGTAACAGCTCCCCCCTTAACTTGCAGGGGAAAGC CAAGAAGCTGTGGAAAGCCGTTTCCATTCTTGGTTCCCTCTGCTGTCCATGGGTACTTGTTCGCTTCCTG AAGCATCTCTTGGTGGCTGTTTTGCTTAGAGAGCAAACAAGGCTGCCCGGGCAGCGTGCTTTAGATGTGG TATTTATTTTCATAGTTCCTATGAACACTTGGGAGGAAGAGCTGAGAAAGTGGACTTTATTTTTCAAATT TGATGTCATAATATTGTGTAAAAAGGAAAGAAACAGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_138650
• Insert Size: 2367 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015278, AAH15278
• RefSeq Size: 3201 bp
• RefSeq ORF: 2367 bp
• Locus ID: 110197
• UniProt ID: Q91WG7
• Cytogenetics: 16 13.37 cM
• Gene Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:32033984). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:32033984). Has no apparent specificity with regard to the acyl compositions of diacylglycerol (By similarity). Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma (PubMed:32033984). Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination (PubMed:32033984).[UniProtKB/Swiss-Prot Function]