Egf (BC017681) Mouse Untagged Clone

CAT#: MC201132

Egf (untagged) - Mouse epidermal growth factor (cDNA clone MGC:18573 IMAGE:4221592), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Egf
• Synonyms: AI790464
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC017681
  AGAAGGGATTCCTATCTGTATATAGGGAAGGAATCCTATCTGCATATTTCGTTGTTAGCACCATCCCTCA TCCCGGTGGGGCTTGGAACTTTCCATCAATTCTTTCCTGTCTCGTTTCTCTTTCATCCTTTGCCTGGTTG TGCCTGTCTCAGGGAGAAATCAGTCACCTGCAGGCCTTGCAGGGCTCTTAGGCTCTGGGAAATTTGTCAT ACGGGTGTCAGGTACTTCTTATTGCTGTCCAAAGGGAAAAAAAAAGTGAGACAAAGAACTCTCCCGGAGC CTTTCCGGCTGCACTCAGAGGCTCTCGAGAGGTGCAGAAGGACCTGGAAAGGCAGCTAAATAAAAGATGC CCTGGGGCCGAAGGCCAACCTGGCTGTTGCTCGCCTTCCTGCTGGTGTTTTTAAAGATTAGCATACTCAG CGAGAGAAGCGGGACTTGTGCCGGTCCTGCCCCCTTCCTAGTTTTCTCACAAGGAAAGAGCATCTCTCGG ATTGACCCAGATGGAACAAATCACCAGCAATTGGTGGTGGATGCTGGCATCTCAGCAGACATGGATATTC ATTATAAAAAAGAGAGACTCTATTGGGTGGATGTAGAAAGACAAGTTTTGCTAAGAGTTTTCCTTAACGG GACAGGACTAGAGAAAGTGTGCAATGTAGAGAGGAAGGTGTCTGGGCTGGCCATAGACTGGATAGATGAT GAAGTTCTCTGGGTAGACCAACAGAACGGAGTCATCACCGTAACAGATATGACAGGGAAAAATTCCCGAG TTCTTCTAAGTTCCTTAAAACATCCGTCAAATATAGCAGTGGATCCAATAGAGAGGTTGATGTTTTGGTC TTCAGAGGTGACCGGCAGCCTTCACAGAGCACACCTCAAAGGTGTTGATGTAAAAACACTGCTGGAGACA GGGGGAATATCGGTGCTGACTCTGGATGTCCTGGACAAACGGCTCTTCTGGGTTCAGGACAGTGGCGAAG GAAGCCACGCTTACATTCATTCCTGTGATTATGAGGGTGGCTCCGTCCGTCTTATCAGGCATCAAGCACG GCACAGTTTGTCTTCAATGGCCTTTTTTGGTGATCGGATCTTCTACTCAGTGTTGAAAAGCAAGGCGATT TGGATAGCCAACAAACACACGGGGAAGGACACGGTCAGGATTAACCTCCATCCATCCTTTGTGACACCTG GAAAACTGATGGTAGTACACCCTCGTGCACAGCCCAGGACAGAGGACGCTGCTAAGGATCCTGACCCCGA ACTTCTCAAACAGAGGGGAAGACCATGCCGCTTCGGTCTCTGTGAGCGAGACCCCAAGTCCCACTCGAGC GCATGCGCTGAGGGCTACACGTTAAGCCGAGACCGGAAGTACTGCGAAGATGTCAATGAATGTGCCACTC AGAATCACGGCTGTACTCTTGGGTGTGAAAACACCCCTGGATCCTATCACTGCACATGCCCCACAGGATT TGTTCTGCTTCCTGATGGGAAACAATGTCACGAACTTGTTTCCTGCCCAGGCAACGTATCAAAGTGCAGT CATGGCTGTGTCCTGACATCAGATGGTCCCCGGTGCATCTGTCCTGCAGGTTCAGTGCTTGGGAGAGATG GGAAGACTTGCACTGGTTGTTCATCGCCTGACAATGGTGGATGCAGCCAGATCTGTCTTCCTCTCAGGCC AGGATCCTGGGAATGTGATTGCTTTCCTGGGTATGACCTACAGTCAGACCGAAAGAGCTGTGCAGCTTCA GGACCACAGCCACTTTTACTGTTTGCAAATTCCCAGGACATCCGACACATGCATTTTGATGGAACAGACT ACAAAGTTCTGCTCAGCCGGCAGATGGGAATGGTTTTTGCCTTGGATTATGACCCTGTGGAAAGCAAGAT ATATTTTGCACAGACAGCCCTGAAGTGGATAGAGAGGGCTAATATGGATGGGTCCCAGCGAGAAAGACTG ATCACAGAAGGAGTAGATACGCTTGAAGGTCTTGCCCTGGACTGGATTGGCCGGAGAATCTACTGGACAG ACAGTGGGAAGTCTGTTGTTGGAGGGAGCGATCTGAGCGGGAAGCATCATCGAATAATCATCCAGGAGAG AATCTCGAGGCCGCGAGGAATAGCTGTGCATCCAAGGGCCAGGAGACTGTTCTGGACGGACGTAGGGATG TCTCCACGGATTGAAAGCGCTTCCCTTCAAGGTTCCGACCGGGTGCTGATAGCCAGCTCCAATCTACTGG AACCCAGTGGAATCACGATTGACTACTTAACAGACACTTTGTACTGGTGTGACACCAAGAGGTCTGTGAT TGAAATGGCCAATCTGGATGGCTCCAAACGCCGAAGACTTATCCAGAACGACGTAGGTCACCCCTTCTCT CTAGCCGTGTTTGAGGATCACCTGTGGGTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGA GGACTGGCCAAAACAGGGTACGTCTTCAAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCATCC ATTGGCAAAACCAGGTGCAGATCCCTGCTTATACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGC CTGGGCACAGCTCGGTGTTTGTGTCGTGAAGGTTTTGTGAAGGCCTGGGATGGGAAAATGTGTCTCCCTC AGGATTATCCAATCCTGTCAGGTGAAAATGCTGATCTTAGTAAAGAGGTGACATCACTGAGCAACTCCAC TCAGGCTGAAGTACCAGACGATGATGGGACAGAATCTTCCACACTAGTGGCTGAAATCATGGTGTCAGGC ATGAACTATGAAGATGACTGTGGTCCCGGGGGGTGTGGAAGCCATGCTCGATGCGTTTCAGACGGAGAGA CTGCTGAGTGTCAGTGTCTGAAAGGGTTTGCCAGGGATGGAAACCTGTGTTCTGATATAGATGAGTGTGT GCTGGCTAGATCGGACTGCCCCAGCACCTCGTCCAGGTGCATCAACACTGAAGGTGGCTACGTCTGCAGA TGCTCAGAAGGCTACGAAGGAGACGGGATCTCCTGTTTCGATATTGACGAGTGCCAGCGGGGGGCGCACA ACTGCGCTGAGAATGCCGCCTGCACCAACACGGAGGGAGGCTACAACTGCACCTGCGCAGGCCGCCCATC CTCGCCCGGACGGAGTTGCCCTGACTCTACCGCACCCTCTCTCCTTGGGGAAGATGGCCACCATTTGGAC CGAAATAGTTATCCAGGATGCCCATCCTCATATGATGGATACTGCCTCAATGGTGGCGTGTGCATGCATA TTGAATCACTGGACAGCTACACATGCAACTGTGTTATTGGCTATTCTGGGGATCGATGTCAGACTCGAGA CCTACGATGGTGGGAGCTGCGTCATGCTGGCTACGGGCAGAAGCATGACATCATGGTGGTGGCTGTCTGC ATGGTGGCACTGGTCCTGCTGCTCCTCTTGGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGC TATCAAACCCCCCAAAGAACCCTTGTGATGAGCCAAGCGGAAGTGTGAGCAGCAGCGGGCCCGACAGCAG CAGCGGGGCAGCTGTGGCTTCTTGTCCCCAACCTTGGTTTGTGGTCCTAGAGAAACACCAAGACCCCAAG AATGGGAGTCTGCCTGCGGATGGTACGAATGGTGCAGTAGTAGATGCTGGCCTGTCTCCCTCCCTGCAGC TCGGGTCAGTGCATCTGACTTCATGGAGACAGAAGCCCCACATAGATGGAATGGGCACAGGGCAAAGCTG CTGGATTCCACCATCAAGTGACAGAGGACCCCAGGAAATAGAGGGAAACTCCCACCTACCCTCCTACAGA CCTGTGGGGCCGGAGAAGCTGCATTCTCTCCAGTCAGCTAATGGATCGTGTCACGAAAGGGCTCCAGACC TGCCACGGCAGACAGAGCCAGTTCAGTAGAAACTGGGAGTAGACAGAAGGTACAGAAGGGAAAATAACAA ACCAGGCTGATGATGGTAGAGTGCTACAGACTTGGTACTCCAGTTTCCACGGCTAATCACTGCTCGCTCA GGGTCCTGAAGATAGCTGCACAGCTGCAGAGCTGCACAGCGGGATAGCTGCGACTTTTGCTTCTTGCTTT AAGCAGTTCCACTGAAGATACTCAAAAGAGAAGTGGAGAAAATCATTAGAAACCAAAGTCAAGACATTCA TATATAAGCTGTGTCTTCTTCACTGGACGGTTTGCCTCTTTTCCTTTTGCCTCAGAAGGAGTGGGTTAAA GCAGGTGACCCCATGCTCTGTCAACCCCTGAATAAATGATGTGATCTACATAGAAGTCTTAGCTCACTCT CAGGAACGCTTGGAACACTATAACTTTTGCTATGATATACTGCCAAGTGTGGCCCATGCTCATAATTGTG CCTTCTGAATTGTGATAAATTAGTGAAAAAACTGTAACTTAGAATCTGATTTATTCAGGATTAGATCATC TTTTTATACTATAAAAATCTTCGAATGAAAATATTTAACTTTAAAAACATTACCTTAATCATTGTCTTTT CTTCTTGAAGTCTTTCCCAGTGAAAACGCTCAATTCTGCTGTTTCCATAGAATTTTTAATTTATTTTAAG ACATGAGATTGTAAACAAATTGCTTGATTTATTTTATCCTAATTATTTAAATAAAATCACCCTAAAGCAT CAGCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC017681
• Insert Size: 3603 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017681, AAH17681
• RefSeq Size: 4709 bp
• RefSeq ORF: 3603 bp
• Locus ID: 13645
• Cytogenetics: 3 58.5 cM
• Gene Summary: This gene encodes epidermal growth factor (EGF), the founding member of the EGF family of growth factors that are implicated in cell proliferation and differentiation. The encoded protein can localize to the membrane and function in juxtacrine signaling or undergo proteolytic processing to generate a soluble form of the hormone. Mice lacking the encoded protein do not exhibit an abnormal phenotype but transgenic mice overexpressing the encoded protein exhibit hypospermatogenesis. [provided by RefSeq, Jul 2016]