Mkln1 (NM_013791) Mouse Untagged Clone

CAT#: MC200957

Mkln1 (untagged) - Mouse muskelin 1, intracellular mediator containing kelch motifs (Mkln1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mkln1
• Synonyms: A130067F06Rik; AU015903; BB154892
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC013703 sequence for NM_013791
  CGGTGCTGACAAGATGGCGGCTGGCGGGGCTGTTGCTGTGGCGCCGGAGTGCCGGCTTCTGCCCTACGCG CTGCATAAGTGGAGCTCCTTCTCCTCCACCTACCTTCCCGAGAACATTTTAGTGGATAAACCAAATGATC AGTCTTCCAGATGGTCTTCAGAGAGCAACTATCCTCCCCAATACTTGATTCTGAAGCTTGAAAGGCCTGC TATCGTTCAGAATATTACATTTGGAAAATATGAGAAAACTCATGTCTGCAATTTGAAGAAATTCAAAGTT TTTGGTGGAATGAATGAAGAAAATATGACAGAGCTGTTGTCCAGTGGCCTGAAGAATGATTATAACAAAG AGACATTTACCTTGAAACATAAAATTGATGAACAGATGTTCCCTTGTCGATTCATTAAAATTGTTCCACT TTTGTCCTGGGGGCCCAGCTTTAACTTTAGCATCTGGTATGTTGAACTTAGTGGCATTGATGATCCTGAT ATCGTGCAGCCCTGTCTGAACTGGTACAGCAAGTACCGTGAACAGGAAGCCATTCGCCTTTGCCTAAAAC ACTTCAGACAACATAACTATACAGAGGCTTTTGAATCCCTGCAGAAGAAAACCAAGATTGCACTGGAACA TCCAATGTTGACAGATATGCATGACAAATTGGTGTTAAAGGGTGACTTTGATGCTTGCGAGGAGTTGATT GAAAAAGCTGTAAATGATGGCTTGTTCAATCAGTATATCAGTCAACAGGAGTATAAGCCACGGTGGAGTC AGATTATTCCTAAGAGCACCAAAGGCGATGGAGAAGATAACAGACCAGGAATGAGAGGGGGCCATCAAAT GGTCATTGACGTCCAGACAGAGACTGTTTATTTGTTTGGTGGCTGGGATGGAACACAAGATCTTGCTGAC TTCTGGGCATACAGTGTGAAGGAGAACCAGTGGACATGTATTTCTAGAGACACTGAGAAAGAGAATGGTC CGAGTGCCAGATCGTGTCATAAGATGTGCATTGACATTCAGCGGAGACAAATCTACACATTGGGCCGTTA CTTGGATTCCTCTGTGAGGAACAGCAAATCTCTGAAAAGTGATTTCTACCGTTACGACATTGACACTAAT ACCTGGATGTTACTAAGTGAAGATACTGCCGCCGACGGTGGACCAAAGTTGGTGTTTGATCATCAGATGT GTATGGACTCAGAAAAACATATGATCTACACGTTTGGTGGCAGGATTTTGACGTGTAATGGCAGTGTAGA TGACAGCAGAGCCAGTGAGCCACAATTCAGTGGGTTGTTTGCGTTCAACTGCCAGTGTCAAACTTGGAAA CTTCTTCGAGAGGATTCCTGTAATGCTGGCCCTGAGGACATCCAGTCTCGGATTGGACACTGCATGCTGT TCCACTCAAAAAACCGTTGCTTATATGTGTTTGGTGGCCAGAGATCCAAGACCTATTTGAATGATTTCTT TAGTTATGATGTGGACTCAGATCATGTAGACATAATTTCAGATGGCACCAAGAAAGACTCTGGAATGGTT CCAATGACCGGATTCACACAGAGAGCAACTATTGATCCAGAACTGAATGAAATACATGTCTTATCCGGAC TTAGCAAAGACAAAGAAAAGAGGGAAGAGAATGTCAGAAATTCATTCTGGATTTATGACATTGTGAGGAA TAGCTGGTCTTGTGTCTATAAGAATGATCAAGCTACAAAAGATAATCTGAGTAAAAGTCTTCAGGAGGAA GAACCATGTCCAAGGTTTGCCCATCAACTCGTTTATGATGAATTACACAAGGTTCATTATTTATTTGGTG GAAATCCAGGAAAATCTTGCTCTCCAAAGATGAGACTAGATGACTTCTGGTCACTGAAGTTGTGTAGACC TTCAAAAGACTACTTACTGAGGCATTGCAAGTACCTCATAAGAAAACACAGGTTTGAAGAAAAGGCCCAA ATGGATCCCCTTAGTGCTCTGAAGTATTTACAAAATGATCTTTATATAACTGTGGATCATTCGGACCCAG AAGAGACAAAAGAGTTTCAACTCCTAGCCTCAGCTCTGTTCAAATCTGGCTCAGATTTCACAGCTCTAGG CTTTTCAGATGTGGATCATACCTATGCTCAAAGAACTCAGCTCTTTGACACCTTAGTAAATTTCTTTCCT GACAGCATGACTCCTCCTAAAGGCAACCTGGTAGACCTGATCACACTGTAGACTGGGGAGTGGGATTGAA GAATTACTGGGCTCAGACATGGAGAAGGAACCAGCTGTTCTGTGGGCAGAGGTGACGCTGCAGGTACTGA GGACGGCACCAGAGTTTTGAAGGATCCTTATTATCACAAGTTTTAACCCTCTTCCTTCATACCTGACCTC AATCCCCTTTTCCTCTTATTTCTGCATTAGGCTAATAAAGTGAAATTGATATACTTCCCATTTAAAAAAA AAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013791
• Insert Size: 2208 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013703, AAH13703
• RefSeq Size: 2460 bp
• RefSeq ORF: 2208 bp
• Locus ID: 27418
• UniProt ID: O89050
• Cytogenetics: 6 A3.3
• Gene Summary: Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (By similarity). Required for internalization of the GABA receptor GABRA1 from the cell membrane via endosomes and subsequent GABRA1 degradation (PubMed:21482357). Acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component THBS1 (PubMed:9724633, PubMed:18710924).[UniProtKB/Swiss-Prot Function]