Rgn (NM_009060) Mouse Untagged Clone

CAT#: MC200804

Rgn (untagged) - Mouse regucalcin (Rgn), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgn
• Synonyms: AI265316; GNL; SMP30
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC012710 sequence for NM_009060
  CCACGCGTCCGTGGAGTTTTTCCTTTGTCTACTATTTTAAAGGTATCTTGAAAAAAAAAAACTTCACTGT CCTTTTCCTGTGACCATGTCTTCCATCAAAGTTGAATGTGTTTTACGGGAGAACTACAGGTGTGGGGAGT CTCCTGTATGGGAGGAAGCGTCACAGTCGCTACTGTTTGTAGATATCCCTTCAAAGATTATTTGTCGATG GGATACGGTCAGCAATCAAGTGCAGCGAGTTGCTGTGGATGCCCCAGTCAGTTCAGTGGCACTTCGACAG TTGGGAGGCTATGTTGCCACCATTGGAACCAAGTTCTGTGCTTTGAACTGGGAAAATCAATCAGTATTTG TCCTAGCCATGGTGGATGAAGATAAGAAAAATAATCGATTCAATGATGGGAAGGTGGATCCTGCTGGGAG ATACTTTGCTGGTACCATGGCTGAGGAAACGGCCCCAGCTGTTCTTGAGCGGCACCAAGGGTCCTTGTAC TCCCTCTTTCCTGATCACAGTGTGAAGAAATACTTTGACCAAGTGGATATCTCCAATGGTTTGGATTGGT CCCTGGACCATAAAATCTTCTACTACATTGACAGCCTGTCCTACACTGTGGATGCCTTTGACTATGACCT ACAAACAGGACAGATTTCCAACCGCAGAATTGTTTACAAGATGGAAAAAGATGAACAAATCCCAGATGGA ATGTGCATTGATGCTGAGGGAAAGCTATGGGTGGCCTGTTACAATGGAGGAAGAGTAATTCGCCTGGATC CTGAGACAGGGAAAAGACTGCAAACTGTGAAGTTGCCTGTTGATAAAACAACTTCATGCTGCTTTGGAGG GAAAGATTACTCTGAAATGTATGTGACCTGTGCCAGGGATGGGTTGAATGCTGAAGGCCTTTTGAGGCAG CCTGATGCTGGTAACATTTTCAAGATAACAGGTCTCGGAGTCAAAGGAATTGCCCCATATTCCTATGCAG GGTGAACTGCAGCTCTTCCTTGCTGTCAGAAGAAAAAGCTTTGAAGATAACTGAAGAATTAAGGGACTGG AATCAATGAACTTTCAATTTAGTTTTTTTTAATGAGGTGGTGATATTATAGCATGGTTAAGCTTTAATTT ACATCTTTGATTGGGCTCTGGTTGAATAAACCTAGGGCATAGCATATTAATGAAGAGTGTCATCCCTGGA TTCCTTTATTTACAATTTTTTAAAGGTCGAAGATTTTCCCCAGAGAATGACAAGTGGTTTTGACAACACA CTCTAGGCCTTCTATTAAAAGCAATTCTGTAGAAATGCAACAGGGAGTTCAACTATCAATCAGCCTGATG ATGGCAAATTGCTCTGGGGGTTGAAATGGGACTGTGACCATTCTTTTTCTGTGTTCCATATTTGCTGCGC ATAAGGCTTCCAAAACAAATCAATGCATAGAAACTTAATGATAATTTATAATGATTATGTGACTTTTCAT GTGTGTTATTTTACAAACTAAAATACTGGCAGAAGATGTTATTCAAACCCTGGGGCACAATCTGTGGTTC TAATTGCCACCTAGTGGCTATACAAATAAATGCATCACATTGCAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_009060
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC012710, AAH12710
• RefSeq Size: 1598 bp
• RefSeq ORF: 900 bp
• Locus ID: 19733
• UniProt ID: Q64374
• Cytogenetics: X A1.3
• Gene Summary: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Catalyzes a key step in ascorbic acid (vitamin C) biosynthesis. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).[UniProtKB/Swiss-Prot Function]