Ppme1 (NM_028292) Mouse Untagged Clone

CAT#: MC200690

Ppme1 (untagged) - Mouse protein phosphatase methylesterase 1 (Ppme1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ppme1
• Synonyms: 1110069N17Rik; 2700017M01Rik; PME-1; Pme1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC014867 sequence for NM_028292
  CTGTGACCGGTTGTACTGCACGTATCGTGGGACGGACCTTGGGCCACTGTTGTCGACGTGCGGCCTCCCT TTGATGTCGGCCCTTGAAAAAAGCATGCACCTCGGCCGCCTACCTTCTCGCCCTCCTCTACCCGGCAGCG GGGGCAGTCAGAGCGGAGCCAAGATGCGGATGGGCCCTGGACGGAAGCGGGACTTTACCCCTGTCCCATG GAGTCAGTACTTTGAGTCAATGGAAGATGTGGAAGTGGAGAATGAAACTGGCAAGGATACTTTTCGAGTT TACAAGAGTGGTTCTGAGGGTCCAGTCCTGCTGCTGCTTCATGGAGGAGGCCATTCCGCCCTTTCCTGGG CAGTGTTCACGGCAGCAATCATCAGTAGAGTACAGTGTAGGATTGTGGCTCTGGATCTGCGAGGTCACGG AGAAACTAAAGTCAAGAATTCTGAAGACCTGTCAGCAGAAACAATGGCAAAAGATGTTGGCAATGTGGTG GAAGCCATGTATGGAGACCTCCCTCCTCCAGTCATGCTGATTGGGCACAGCATGGGTGGCGCCATTGCAG TGCACACAGCCGCAGCTAACCTGGTACCAAGCCTCTTGGGCCTTTGCATGATTGATGTTGTAGAAGGTAC CGCCATGGATGCACTTAATAGCATGCAGAATTTCTTGCGTGGTCGACCTAAAACCTTCAAGTCTTTGGAG AATGCTATTGAATGGAGTGTGAAGAGTGGCCAGATTCGAAACCTTGAGTCTGCCCGTGTATCCATGGTCG GCCAAGTCAAACAGTGTGAAGGAATTACAAGTCCAGAAGGTTCCAAATCCATAGTGGAAGGAATCATAGA GGAGGAGGAAGAAGATGAGGAAGGAAGTGAGTCAGTTAACAAGAGGAAAAAGGAAGACGACATGGAAACC AAGAAGGATCACCCATACACCTGGAGAATTGAGCTGGCAAAAACAGAAAAGTACTGGGATGGCTGGTTCC GGGGCTTATCCAATCTCTTTCTTAGCTGTCCTATTCCTAAACTGCTGCTCTTGGCAGGTGTTGACAGATT GGATAAAGATCTGACCATAGGCCAGATGCAGGGGAAGTTCCAGATGCAGGTCTTACCCCAGTGTGGCCAT GCAGTCCATGAGGATGCCCCTGACAAGGTAGCTGAAGCTGTTGCCACTTTCCTGATCCGGCACAGGTTTG CAGAGCCCATTGGAGGATTCCAGTGTGTGTTTCCTGGCTGCTAGTGACCTGCTGTCTACTCCTCCCTCTA CATTGAGCTCTGTTGTAAATACATCGCACCAGAGGCCACTGTGACGCCGCTGTCTCCTCCTCTCCATCCC GCCCAGCCATGTGACACCGGCTCTTGTAGAGGGCATCCCCAGATGTCCAAACCCTTTCCTGTGTACTGTT GAAAGCATTGTTCTTCAGGGCCCTTGTCCAACAGTGGCCCGTGCAGTCTGGGGTCCACAGCTCTTCCTCT CCTTCCTGTGCTCCCTGCCTTGCCTAGGATGAAGCCTCCAGCGCTGCTCCCTGGCCCTGTTCCTGGCATA TGGCAATGTACCCCAGGCTCAGGGATCTCCCTTCCTTGAGGATGTTCTTGGCATGGTCCTGCCCTACCTC ATGGGATGGGCAATGCACACACTGGCCCTTATTTTTCCCTTTCAAATAAAACACCAGTCAGGTACCTTTA TCCCAGTCTTAACTGTCCCAAATCTGGAAGGTCCAGAGTAAGCAGGATTCAGGGAGAGGGAGTGGATAGC AAGTATCCCAAGAAACCAACCTGTAAGTCAGGTCCAGCCAGTCCAAGCACATGGCTTCCCATCTGGGTGA GCCCACTGTCCCACTCCCACATGTCTGGGCACCTGCCCTGGGCTGAGGCCAGGCTGCTCCAAGGGCCGCA TGAGCCCTAATCTGCCACAGAGCAACCCAGGTTAAACACAGCCCATGCACAAAGCCACAGGCTAAATCCT GTGGAATTGTTTTTAATGACTGAATTTAACCATTTTCATAGTTGGTTCCTGGAGGTGTGCCAAGTGCCCG CTTGCCTCTTCTAGACCCACAGCTTCTTGATCCACTTGTGGTTTCCATGTCACTAATGTAGAAACATCAT GGACTAGCATCCCCAGTCTTTGCCCTCATCCAGCTGTCGCAGTGCAGCACTGGGGCCTCCCCCTGCTGCC CATGGGGGGGGGGGGTGGGCAGCCTCCTGAAACCCATCTTTCTGTGACTGTCTTAGGTGACGTGTAGCCC TCTTCCATTTTTTCACCCAACAACTTCCTCTGTCCTGCTGCACGGTCCAGAGTCTGGGACCGACTTTGTT TCTTTGTTATTTATGATCTTGTTTAAAGAAAATAAATATCTCCCAACCTTTAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028292
• Insert Size: 1161 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014867, AAH14867
• RefSeq Size: 2376 bp
• RefSeq ORF: 1161 bp
• Locus ID: 72590
• UniProt ID: Q8BVQ5
• Cytogenetics: 7 E2
• Gene Summary: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme (By similarity).[UniProtKB/Swiss-Prot Function]