Exo1 (NM_012012) Mouse Untagged Clone

CAT#: MC200610

Exo1 (untagged) - Mouse exonuclease 1 (Exo1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Exo1
• Synonyms: 5730442G03Rik; Msa
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC006671 sequence for NM_012012
  GGCTGCGCTTGCAGCAGCCGCTCGCCGCCGTTCTGAACCCCAGGTTTGAGGAGCCACGGCGCCTGGAATG GACTTGTGTGCTATGCCTGTTCGTTCGGGAGACGGGACTAAGTTAACTTGGCACGATGGGGATTCAAGGG TTACTTCAGTTCATCCAAGAAGCTTCTGAACCTGTCAACGTGAAGAAGTACAAAGGACAGGCAGTGGCTG TGGATACCTACTGTTGGCTTCACAAAGGGGCTATTGCTTGTGCTGAAAAGCTAGCCAAAGGGGAACCTAC AGATAGGTATGTAGGATTTTGTATGAAGTTTGTTAATATGCTGCTGTCTTATGGGGTCAAGCCGATTCTC ATATTTGACGGATGTACTCTACCTTCTAAAAAGGAAGTGGAGCGGTCTCGAAGAGAGAGACGACAAAGCA ACCTTCTTAAAGGGAAGCAGCTTCTTCGAGAGGGCAAAGTGTCAGAAGCCCGAGACTGCTTCGCTCGCTC TATCAACATCACGCACGCCATGGCCCACAAAGTAATAAAAGCTGCTCGGGCCCTAGGAGTGGATTGCCTC GTGGCTCCGTATGAAGCTGATGCTCAGTTGGCCTACCTTAACAAGGCTGGCATCGTGCAGGCTGTCATCA CAGAGGACTCTGACCTCCTCGCATTTGGCTGTAAGAAGGTGATTTTAAAAATGGATCAGTTTGGAAATGG ACTGGAAGTGGACCAGGCACGGCTAGGCATGTGCAAGCAGCTTGGGGATGTATTCACGGAGGAGAAGTTC CGGTACATGTGCATTCTGTCCGGCTGTGACTACCTCGCCTCCCTTCGTGGGATCGGCTTAGCCAAGGCCT GCAAAGTGCTGAGACTGGCCAATAACCCCGATATCGTGAAGGTTATCAAGAAAATTGGGCATTATCTCAG GATGAATATAACGGTGCCCGAGGATTACATCACAGGATTTATTCGTGCCAACAATACTTTCCTCTACCAG CTCGTGTTCGACCCCATCCAAAGGAAGCTGGTCCCTCTGAATGCCTACGGAGATGACGTTAATCCCGAAA CACTGACTTACGCTGGGCAGTACGTCGGTGACTCTGTAGCTCTTCAGATCGCCCTTGGAAATAGAGATGT AAATACTTTTGAACAGATTGATGACTACAGTCCAGACACCATGCCAGCCCACTCAAGAAGCCACAGCTGG AATGAGAAAGCAGGTCAGAAACCACCTGGTACCAACAGCATTTGGCACAAGAATTATTGTCCTAGACTTG AGGTGAACAGTGTCTCCCACGCTCCTCAACTGAAGGAAAAGCCAAGCACTTTGGGCCTTAAACAAGTGAT TAGTACTAAAGGGTTAAATCTTCCCAGGAAGTCTTGTGTGTTGAAAAGACCAAGAAATGAAGCGCTGGCT GAAGATGACCTGTTGAGCCAGTATTCGTCAGTTTCAAAGAAGATCAAGGAAAATGGCTGTGGGGATGGCA CATCACCTAACTCTTCTAAAATGTCCAAGTCCTGCCCCGATTCTGGGACTGCTCACAAGACAGATGCACA CACCCCGTCTAAGATGAGGAATAAATTTGCAACGTTCTTACAGAGGAGGAATGAAGAAAGCGGTGCAGTC GTGGTTCCAGGGACCAGAAGCAGGTTTTTTTGCAGTTCTCAGGATTTTGACAATTTCATACCAAAAAAAG AAAGCGGCCAGCCTCTAAATGAAACTGTGGCCACTGGCAAAGCCACCACCAGCCTCCTCGGGGCACTGGA CTGTCCAGACACGGAAGGCCACAAGCCGGTTGATGCAAATGGGACGCACAATCTGAGCTCTCAGATTCCA GGCAATGCAGCTGTGTCTCCTGAGGATGAGGCTCAGTCCTCTGAGACCAGCAAACTCTTGGGGGCCATGT CCCCACCCAGTCTGGGGACACTAAGAAGCTGTTTCAGTTGGTCTGGCACTCTCAGGGAATTTTCTCGAAC TCCAAGTCCCTCAGCAAGCACTACATTGCAGCAGTTCCGGAGAAAGAGCGACCCCCCTGCCTGTCTTCCT GAGGCCTCTGCCGTGGTCACTGACAGGTGCGACTCAAAGAGCGAGATGCTGGGTGAGACGTCTCAGCCCT TGCACGAGCTGGGCTGCTCCTCGCGGTCTCAGGAAAGCATGGACTCCTCGTGCGGCCTAAACACGTCGAG CCTGTCCCAGCCTTCCAGTAGGGATTCAGGTTCAGAGGAGTCGGATTGTAACAATAAGTCACTTGATAAT CAAGGGGAACAGAACTCCAAGCAACACTTACCTCACTTTTCAAAGAAAGACGGACTTAGACGGAACAAGG TTCCTGGGCTCTGTAGGTCTAGTTCTATGGACTCTTTTTCTACAACCAAGATCAAGCCCCTGGTGCCTGC CAGGGTCAGTGGCCTGAGCAAGAAGTCAGGAAGCATGCAGACGAGAAAACACCATGATGTGGAGAACAAG CCAGGCCTACAGACGAAGATCAGCGAGCTCTGGAAAAACTTTGGGTTTAAAAAAGATTCTGAAAAGCTTC CTTCTTGTAAGAAGCCCCTGTCTCCAGTCAAGGACAATATCCAACTCACTCCAGAGACAGAAGATGAGAT CTTTAACAAGCCCGAGTGTGTACGCGCTCAGAGAGCAATATTTCACTAAGTGCAGACCGTTGCCTACAAG AAAATCTTATCAACTGGAGAGATGTATTAGTCTCAATGTCATTTTTTAAATGGATTACATTTTGTATATT AACTTTGTGACTTTCTTTTGCTCAGCTTTTTATATTTTCATAGAAAGCTAAATAGAAGAATAAATCTGTC TTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_012012
• Insert Size: 2514 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC006671, AAH06671
• RefSeq Size: 2847 bp
• RefSeq ORF: 2514 bp
• Locus ID: 26909
• UniProt ID: Q9QZ11
• Cytogenetics: 1 81.9 cM
• Gene Summary: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.[UniProtKB/Swiss-Prot Function]