Ilk (NM_010562) Mouse Untagged Clone

CAT#: MC200535

Ilk (untagged) - Mouse integrin linked kinase (Ilk), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ilk
• Synonyms: AA511515; ESTM24
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003737 sequence for NM_010562
  CCCCAGTCCCGGGGTCTCTGTGCCGGGACGCTGCTATGGACGACATTTTCACTCAGTGCCGGGAGGGCAA CGCGGTGGCGGTGCGCTTGTGGCTGGACAACACAGAGAACGACCTCAATCAGGGGGATGATCATGGCTTC TCCCCCTTGCACTGGGCCTGCCGAGAAGGCCGCTCTGCGGTGGTTGAAATGCTGATCATGCGTGGAGCAC GGATTAATGTGATGAATCGTGGGGATGATACCCCCCTGCACCTGGCAGCTAGTCATGGACACCGTGACAT TGTACAGAAGCTGTTGCAATACAAGGCTGACATCAATGCAGTGAATGAGCACGGCAATGTGCCACTTCAC TATGCATGTTTCTGGGGCCAAGACCAGGTGGCAGAGGACCTGGTGGCTAACGGGGCTCTTGTGAGCATCT GTAACAAGTATGGAGAGATGCCTGTGGACAAAGCCAAGGCACCCCTTAGAGAGCTTCTCCGAGAACGGGC AGAGAAAATGGGCCAGAATCTCAACCGTATTCCATACAAGGACACATTCTGGAAGGGGACCACTCGCACA AGGCCCCGAAATGGGACCCTGAACAAACACTCCGGTATTGACTTCAAACAGCTCAACTTTCTGGCAAAGC TCAATGAGAATCATTCTGGAGAGCTTTGGAAAGGCCGCTGGCAGGGCAATGATATTGTTGTGAAGGTGCT GAAGGTTCGAGACTGGAGTACAAGGAAAAGCAGGGACTTCAATGAGGAATGTCCCCGGCTCAGGATTTTC TCACACCCTAACGTGCTCCCAGTGCTAGGTGCTTGCCAGGCTCCCCCAGCCCCCCACCCAACCCTCATCA CACACTGGATGCCATATGGATCTCTCTACAATGTTCTACATGAAGGCACCAATTTCGTTGTGGACCAGAG CCAAGCTGTAAAGTTTGCTTTGGACATGGCAAGAGGCATGGCTTTTCTTCACACACTAGAGCCTCTCATA CCCCGGCATGCACTCAATAGCCGCAGTGTAATGATCGATGAAGATATGACTGCCCGAATCAGCATGGCTG ATGTTAAGTTTTCTTTCCAGTGCCCTGGGCGCATGTATGCGCCTGCCTGGGTGGCCCCTGAAGCCCTGCA GAAGAAGCCTGAAGACACAAACAGACGCTCAGCAGACATGTGGAGTTTCGCGGTGCTTCTGTGGGAACTG GTGACACGAGAGGTGCCCTTTGCTGACCTCTCTAATATGGAGATTGGGATGAAGGTGGCACTGGAAGGCC TTCGGCCTACCATCCCACCAGGTATTTCCCCCCATGTGTGTAAGCTCATGAAGATTTGCATGAATGAAGA CCCTGCAAAGCGACCCAAGTTTGACATGATTGTGCCTATCTTGGAGAAGATGCAGGACAAGTAGGACCTT CAGGTCCTTGCCCAAACTCCAGAAGTGTCAAAACATGGCTGGGGGAGTACACTTCTCCAAAGTTAAAGAC CTCTGGTTGCCTCCCCTGTCTCTAATCATGGTACTACCCCAGTTATGGGACTTGTTCCCTGCTCCCATCC CTACCACTGTAGCCCCAAAAGGGGCTGGGCTCAGAGCTTTGTCACTTGCCACATGATGTCTCCCAACATG GGAGGGATCAGCCCCGCCTGTCACAATAAAGTTTATTATGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010562
• Insert Size: 1359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003737, AAH03737
• RefSeq Size: 1673 bp
• RefSeq ORF: 1359 bp
• Locus ID: 16202
• UniProt ID: O55222
• Cytogenetics: 7 E3
• Gene Summary: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.