Pcyt2 (NM_024229) Mouse Untagged Clone

CAT#: MC200420

Pcyt2 (untagged) - Mouse phosphate cytidylyltransferase 2, ethanolamine (Pcyt2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcyt2
• Synonyms: 1110033E03Rik; ET
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008276 sequence for NM_024229
  GCGGGAGCCGCCAGGATTTGCGGGGGGCCTCCGGTGCCATGATCCGGAACGGGCACGGGGCTGCCAGCGC TGCTGGGCTGAAGGGTCCGGGAGATCAGCGCATCGTGCGGGTGTGGTGCGATGGCTGCTATGACATGGTG CATTATGGCCACTCCAACCAGCTACGGCAGGCACGGGCCATGGGGGACTACCTCATCGTGGGTGTGCATA CGGATGAGGAGATTGCCAAGCATAAGGGGCCCCCGGTGTTTACCCAGGAGGAGAGGTACAAGATGGTACA GGCCATCAAGTGGGTGGATGAGGTGGTGCCCGCTGCTCCCTACGTCACCACCCTGGAGACACTGGACAAG CACAACTGTGACTTCTGTGTTCACGGCAATGACATCACGCTGACAGTAGACGGCCGAGACACCTATGAGG AAGTGAAGCAGGCTGGGAGGTACAGAGAGTGCAAACGCACCCAGGGTGTGTCCACCACAGACCTCGTGGG TCGCATGCTGCTGGTGACCAAGGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAATATGCT GACAGTTTTGGAAAGCCCCCTCACCCGACACCTGCCGGGGACACACTTTCCTCAGAAGTCTCCTCCCAGT GCCCTGGGGGGCAGAGCCCCTGGACAGGGGTGTCCCAGTTTCTACAGACATCCCAGAAGATCATCCAGTT TGCTTCTGGGAAGGAGCCCCAGCCCGGGGAGACGGTCATCTATGTGGCTGGTGCCTTTGACCTGTTCCAC ATCGGGCACGTGGACTTCCTACAGGAGGTGCACAAGCTAGCCAAGAGGCCCTACGTCATCGCCGGCCTAC ACTTTGACCAGGAAGTAAACCGGTACAAGGGCAAGAACTACCCCATCATGAACCTGCACGAGCGGACTCT CAGTGTGCTGGCCTGCCGGTATGTTTCAGAAGTGGTGATTGGGGCACCATACTCCGTGACAGCGGAGCTC CTGAATCACTTCAAGGTGGACCTGGTGTGTCACGGGAAGACAGAGATTGTACCCGACAGGGATGGGTCTG ACCCCTACCAGGAGCCCAAGAGAAGAGGCATCTTCTATCAGATTGACAGTGGCAGTGACCTCACTACAGA CCTGATTGTGCAGAGGATCATCAAGAACAGGCTGGAGTATGAAGCACGGAATCAGAAGAAAGAAGCCAAG GAATTAGCCTTTCTGGAGGCCACGAAGCAGCAGGAGGCGCCGCCTGGAGGGGAGATTGACTAGCTTCAGA CCCGGAGATGTTGTTCACGCCCTTGCCCTGTGCCCACCTCTTCTCTTCCTGCCCGGCTCTGCTTCTGTGT CTTGATGTCAGCTCACACAATTCCAAAGGAAACTGGCCTTGCTGAGGGTGCTGCCTGCCTGCGAGGCACC TGCCTTGCAGCAGGCTCTCAGCCCTTCCCTCTGAGCTGCTCAGAGAGGGTAGCTAGCCCAATGATGTGGC CCGTGGACAGGATAAGCAGATGCACCTGTGACTGACTGGAGGCGTGTTGCTGTGTATCCCGGGCATCCAT GGGTTCCAACTGCCACTGCCCCAGTCTTGGCCAGAGATACCCCTCCTGCCTGACTGGAAGCTGCACATCT GCCCACTGAGCTTTGGTGAAAGGTCCAGAGGCTTTGGGGACCTCTGTTCCTGGGCCACCCTGCCCGTGGG CACCCTCTACCTTGGGGCACGTTCTAGCACCCCATTCCTGACTCCTGGAAGATGCACTTGCCCCGACAGC TGGGCAGCACGGCTGTCCTCTGCAGAGACTGCCTGGTCCTCATTGTACTTTGGTGGCTCAACTGAATAAA GCCTTGTGGGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_024229
• Insert Size: 1215 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC008276, AAH08276
• RefSeq Size: 1908 bp
• RefSeq ORF: 1215 bp
• Locus ID: 68671
• UniProt ID: Q922E4
• Cytogenetics: 11 E2
• Gene Summary: Plays an important role in the biosynthesis of the phospholipid phosphatidylethanolamine. Catalyzes the formation of CDP-ethanolamine (By similarity).[UniProtKB/Swiss-Prot Function]