Tmed9 (BC004691) Mouse Untagged Clone

CAT#: MC200132

Tmed9 (untagged) - Mouse transmembrane emp24 protein transport domain containing 9 (cDNA clone MGC:7860 IMAGE:3501295), complete, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmed9
• Synonyms: 2400003B06Rik
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC004691
  CGGACGCGTGGGCGGACGCGTGGGTGGCTGCGGTGCGAGGCGTGCGGGTTGTCGGAAGCTCACCGGGTCT CTTGCTGGGGAGAGGGATGCGGGCCTTCCTGCTACTGCTGTGGCTTGCAGCCCGCGGAAGCGCGCTGTAT TTCCACATCGGAGAAACAGAAAAGAAGTGCTTCATTGAGGAGATTCCGGACGAGACCATGGTCATCGGAA ATTACCGGACGCAGCTGTATGACAAACAGCGGGAGGAGTACCAGCCAGCCACCCCTGGGCTTGGCATGTT CGTAGAGGTAAAAGACCCCGAGGACAAGGTCATCCTGGCCCGGCAGTATGGCTCTGAGGGCAGGTTCACC TTCACCTCCCACACCCCTGGTGAGCACCAGATCTGTCTTCACTCAAATTCCACCAAGTTCTCCCTCTTTG CTGGAGGGATGCTGAGGGTTCACCTTGACATCCAAGTGGGCGAACATGCAAACGACTATGCAGAAATTGC TGCCAAGGACAAGCTGAGCGAGCTGCAGCTGAGGGTGCGACAGCTGGTGGAGCAAGTGGAGCAGATCCAA AAGGAGCAGAACTACCAGCGGTGGCGAGAAGAGCGCTTCAGACAAACCAGCGAAAGCACTAACCAGCGAG TGCTATGGTGGTCCGTCCTGCAGACTCTCATCCTCCTGGCCATCGGCGTCTGCCAGATGCGGCACCTCAA GAGCTTTTTTGAAGCCAAGAAGTTGGTGTAGCAGCTGTCCCAAGCCTCACAGGCGATCTTTCTTCCAGGC TGTGGGAAGTAGGAGGAGGACTGGTTTTCAGCCGCAGATGTTCTGGAGGAGAGAGGGACTGTAAGCGTGT CCCCGTTCCTCAGGCTCAGGTGGAGGACAGCAGCCTGGCTGGCTAATGCTGAGCCAGCAGTGGGACAAGC CCTCCCACCTCCACTCCTGGACCTTTTGCAGTAATCACTCAGGGGCTGGCGATGCCCTTGGTGTCTCAGA AGCTCAGTGTTACTGATCAGGGATATGAGGTTGCTGTCTGGGGTAGGTTTTGGGAGGGAAGTGGGTGGGG CAGCAAGCTGATTTTCCTTCTGTCTTCCTTTGTTAATGTGGGATTTTGAGCAGGTCAGGGTGTATCAGTG ACTCCTTGCTATTCCAGAAACTTTGCTTTTCTTGCCTTTGGCTTAGAACCAGCCTGCTTATATAGGGTGT GTTTGCTGCGTGTCAGTTTAAGGGTGTGGAGGGGTTGCAGTGCACAAAAGGGGCCCAGAGGTTGATTTTA GTTTGTTACAATTGTCCTTTCTGGGGCTTGAACAACTGGTATCAAACCAAGCAGAGACTTTGGTGCTGGT TGGAAACAGAGCCTACAGAGTCTTAGTTACTGAGTTTATTTTCAATGAATGTGGGTTTGTTACATTGGTT TCCCAATTAAAAAGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: BC004691
• Insert Size: 645 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC004691, AAH04691
• RefSeq Size: 1429 bp
• RefSeq ORF: 645 bp
• Locus ID: 67511
• Cytogenetics: 13 B1
• Gene Summary: Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 (By similarity).[UniProtKB/Swiss-Prot Function]