Nfix (NM_001081982) Mouse Untagged Clone

CAT#: MC200098

Nfix (untagged) - Mouse nuclear factor I/X (Nfix), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nfix
• Synonyms: CTF; NF-I/X; NF1-X; NFI-X
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003766 sequence for NM_001081982
  CGGCCGGCCTCCGCGCTCGCGCCCGGGCGCCCAGCGATGTACTCCCCGTACTGCCTCACCCAGGATGAGT TCCACCCGTTTATCGAGGCGCTGCTGCCTCACGTCCGAGCCTTCTCCTACACCTGGTTCAACCTGCAGGC GCGGAAGCGCAAGTACTTCAAGAAGCACGAGAAGCGGATGTCAAAGGACGAGGAGCGCGCAGTGAAGGAC GAGCTGCTGGGCGAGAAGCCTGAGATCAAGCAGAAGTGGGCATCCCGGCTGTTGGCCAAGCTGCGCAAAG ACATCCGGCCCGAGTTCCGCGAGGACTTTGTGCTAACCATCACGGGCAAGAAGCCCCCCTGCTGCGTGCT TTCCAACCCCGACCAGAAGGGCAAGATCCGGCGGATTGACTGCCTGCGCCAGGCTGACAAGGTGTGGCGG CTGGACCTGGTCATGGTGATTTTGTTTAAAGGGATCCCTTTGGAAAGTACTGATGGGGAGCGGCTCTACA AGTCGCCCCAGTGCTCGAACCCCGGCCTGTGTGTCCAGCCACATCACATTGGAGTCACAATCAAAGAACT GGACCTTTATCTGGCTTACTTTGTCCACACTCCGGAATCCGGACAATCAGATAGTTCAAACCAGCAAGGA GATGCGGACATCAAACCACTGCCCAACGGGCACTTAAGTTTCCAGGACTGCTTTGTGACGTCTGGGGTCT GGAATGTGACAGAGCTGGTGAGAGTATCACAGACTCCAGTTGCGACTGCATCAGGGCCCAACTTCTCACT GGCGGACCTGGAGAGCCCCAGCTACTACAACATAAATCAAGTGACCCTGGGAAGGCGGTCCATCACCTCC CCTCCTTCCACCAGCAGCACCAAGCGCCCCAAGTCCATCGACGACAGTGAGATGGAGAGTCCAGTAGATG ATGTGTTCTATCCTGGGACAGGCCGCTCTCCGGCCGCTGGCAGCAGCCAGTCTAGCGGATGGCCCAATGA CGTGGATGCAGGCCCTGCTTCTCTAAAGAAGTCAGGAAAGCTGGACTTCTGCAGCGCCCTCTCCTCTCAG GGCAGTTCCCCACGCATGGCTTTCACCCACCACCCGCTGCCTGTGCTTGCTGGAGTCAGACCAGGGAGCC CCCGGGCCACGGCATCAGCGCTGCACTTCCCTTCCACGTCCATCATCCAGCAGTCGAGCCCGTATTTCAC ACACCCAACCATCCGCTACCACCACCACCATGGGCAGGACTCGCTGAAGGAGTTTGTGCAGTTTGTGTGC TCTGACGGCTCGGGTCAGGCCACCGGACAGCATTCACAACGACAGGCGCCTCCTCTGCCAACCGGTTTGT CAGCATCGGATCCCGGGACGGCAACTTTCTGAACATCCCACAGCAGTCTCAGTCCTGGTTCCTCTGATTA AGATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_001081982
• Insert Size: 1326 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003766, AAH03766
• RefSeq Size: 1444 bp
• RefSeq ORF: 1326 bp
• Locus ID: 18032
• UniProt ID: P70257
• Cytogenetics: 8 41.02 cM
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Isoform NFIX1 acts as a transcriptional activator while isoform NFIX3 acts as a repressor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, initiates translation at an alternate start codon, and has multiple coding region differences, compared to variant 3. The encoded isoform (1) has a distinct N-terminus and is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.