Human DNASE2B (NM_021233) AAV Particle

CAT#: RC212192A1V

AAV ORF Particles, serotype AAV-2, DNASE2B (Myc-DDK-tagged)-Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: DNASE2B
• Synonyms: DLAD
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212192 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCAAGACTGCTAAGAACATCCTTTGCTTTGCTCTTCCTTGGCCTCTTTGGGGTGCTGGGGGCAG
  CAACAATTTCATGCAGAAATGAAGAAGGGAAAGCTGTGGACTGGTTTACTTTTTATAAGTTACCTAAAAA
  ACAAAACAAGGAAAGTGGAGAGACTGGGTTAGAGTACCTGTACCTAGACTCTACAACTAGAAGCTGGAGG
  AAGAGTGAGCAACTAATGAATGACACCAAGAGTGTTTTGGGAAGGACATTACAACAGCTATATGAAGCAT
  ATGCCTCTAAGAGTAACAACACAGCCTATCTAATATACAATGATGGAGTCCCTAAACCTGTGAATTACAG
  CAGAAAGTATGGACACACCAAAGGTTTACTGCTGTGGAACAGAGTTCAAGGGTTCTGGCTGATTCATTCC
  ATCCCTCAGTTTCCTCCAATTCCGGAAGAAGGCTATGATTATCCACCCACAGGGAGACGAAATGGACAAA
  GTGGCATCTGCATAACTTTCAAGTACAACCAGTATGAGGCAATAGATTCTCAGCTCTTGGTCTGCAACCC
  CAACGTCTATAGCTGCTCCATCCCAGCCACCTTTCACCAGGAGCTCATTCACATGCCCCAGCTGTGCACC
  AGGGCCAGCTCATCAGAGATTCCTGGCAGGCTCCTCACCACACTTCAGTCGGCCCAGGGACAAAAATTCC
  TCCATTTTGCAAAGTCGGATTCTTTTCTTGACGACATCTTTGCAGCCTGGATGGCTCAACGGCTGAAGAC
  ACACTTGTTAACAGAAACCTGGCAGCGAAAAAGACAAGAGCTTCCTTCAAACTGCTCCCTTCCTTACCAT
  GTCTACAATATAAAAGCAATTAAATTATCACGACACTCTTATTTCAGTTCTTATCAAGATCACGCCAAGT
  GGTGTATTTCCCAAAAGGGCACCAAAAATCGCTGGACATGTATTGGAGACCTAAATCGGAGTCCACACCA
  AGCCTTCAGAAGTGGAGGATTCATTTGTACCCAGAATTGGCAAATTTACCAAGCATTTCAAGGATTAGTA
  TTATACTATGAAAGCTGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212192 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMARLLRTSFALLFLGLFGVLGAATISCRNEEGKAVDWFTFYKLPKKQNKESGETGLEYLYLDSTTRSWR
  KSEQLMNDTKSVLGRTLQQLYEAYASKSNNTAYLIYNDGVPKPVNYSRKYGHTKGLLLWNRVQGFWLIHS
  IPQFPPIPEEGYDYPPTGRRNGQSGICITFKYNQYEAIDSQLLVCNPNVYSCSIPATFHQELIHMPQLCT
  RASSSEIPGRLLTTLQSAQGQKFLHFAKSDSFLDDIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYH
  VYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLV
  LYYESCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_021233
• ORF Size: 1071 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_021233.2
• RefSeq Size: 1279 bp
• RefSeq ORF: 1086 bp
• Locus ID: 58511
• UniProt ID: Q8WZ79
• Cytogenetics: 1p31.1-p22.3
• MW: 41.2 kDa