DNASE2B (NM_021233) Human Tagged ORF Clone

USD 460.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name DNASE2B (NM_021233) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol DNASE2B
Synonyms DLAD
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>RG212192 representing NM_021233
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCAAGACTGCTAAGAACATCCTTTGCTTTGCTCTTCCTTGGCCTCTTTGGGGTGCTGGGGGCAG
CAACAATTTCATGCAGAAATGAAGAAGGGAAAGCTGTGGACTGGTTTACTTTTTATAAGTTACCTAAAAA
ACAAAACAAGGAAAGTGGAGAGACTGGGTTAGAGTACCTGTACCTAGACTCTACAACTAGAAGCTGGAGG
AAGAGTGAGCAACTAATGAATGACACCAAGAGTGTTTTGGGAAGGACATTACAACAGCTATATGAAGCAT
ATGCCTCTAAGAGTAACAACACAGCCTATCTAATATACAATGATGGAGTCCCTAAACCTGTGAATTACAG
CAGAAAGTATGGACACACCAAAGGTTTACTGCTGTGGAACAGAGTTCAAGGGTTCTGGCTGATTCATTCC
ATCCCTCAGTTTCCTCCAATTCCGGAAGAAGGCTATGATTATCCACCCACAGGGAGACGAAATGGACAAA
GTGGCATCTGCATAACTTTCAAGTACAACCAGTATGAGGCAATAGATTCTCAGCTCTTGGTCTGCAACCC
CAACGTCTATAGCTGCTCCATCCCAGCCACCTTTCACCAGGAGCTCATTCACATGCCCCAGCTGTGCACC
AGGGCCAGCTCATCAGAGATTCCTGGCAGGCTCCTCACCACACTTCAGTCGGCCCAGGGACAAAAATTCC
TCCATTTTGCAAAGTCGGATTCTTTTCTTGACGACATCTTTGCAGCCTGGATGGCTCAACGGCTGAAGAC
ACACTTGTTAACAGAAACCTGGCAGCGAAAAAGACAAGAGCTTCCTTCAAACTGCTCCCTTCCTTACCAT
GTCTACAATATAAAAGCAATTAAATTATCACGACACTCTTATTTCAGTTCTTATCAAGATCACGCCAAGT
GGTGTATTTCCCAAAAGGGCACCAAAAATCGCTGGACATGTATTGGAGACCTAAATCGGAGTCCACACCA
AGCCTTCAGAAGTGGAGGATTCATTTGTACCCAGAATTGGCAAATTTACCAAGCATTTCAAGGATTAGTA
TTATACTATGAAAGCTGTAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG212192 representing NM_021233
Red=Cloning site Green=Tags(s)

MMARLLRTSFALLFLGLFGVLGAATISCRNEEGKAVDWFTFYKLPKKQNKESGETGLEYLYLDSTTRSWR
KSEQLMNDTKSVLGRTLQQLYEAYASKSNNTAYLIYNDGVPKPVNYSRKYGHTKGLLLWNRVQGFWLIHS
IPQFPPIPEEGYDYPPTGRRNGQSGICITFKYNQYEAIDSQLLVCNPNVYSCSIPATFHQELIHMPQLCT
RASSSEIPGRLLTTLQSAQGQKFLHFAKSDSFLDDIFAAWMAQRLKTHLLTETWQRKRQELPSNCSLPYH
VYNIKAIKLSRHSYFSSYQDHAKWCISQKGTKNRWTCIGDLNRSPHQAFRSGGFICTQNWQIYQAFQGLV
LYYESCK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_021233
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021233.2, NP_067056.2
RefSeq Size 1279
RefSeq ORF 1086
Locus ID 58511
Cytogenetics 1p31.1-p22.3
Protein Families Transmembrane
Protein Pathways Lysosome
Gene Summary The protein encoded by this gene shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this gene product is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

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