HIF1AN (NM_017902) Human 3' UTR Clone

CAT#: SC222457

3' UTR clone of hypoxia inducible factor 1 alpha subunit inhibitor (HIF1AN) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HIF1AN
• Synonyms: FIH1
• ACCN: NM_017902
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222457 3’UTR clone of NM_017902
  The sequence shown below is from the reference sequence of NM_017902. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AACACAATGATCAAGGGCCGATACAACTAGCCTGCCAGGGGTCAAGGCCTCCTGCCAGGTGACTGCTAT
  CCCGTCCACACCGCTTCATTGATGAGGACAGGAGACTCCAAGCGCTAGTATTGCACGCTGCACTTAATG
  GACTGGACTCTTGCCATGGCCCAGGAGTCAGGTGTTTGGAGCGAGGCAGGGCAGTTGGCACTCCACTCC
  TATTTGGAGGGACTTCATACCCTTGCCTCTTGTGCCCCAGCACCTTCTCTCTCTGCCCCCCGCCTAAAG
  TCCTGCATTCAGTGTGTGGAGTCCCAGCTTTTGGTTGTCATCATGTCTGTGTGTATGTTAGTCTGTCAA
  CTTCGGAATGTGTGCGTGTGTGTGCATGCACACGCATGTATGTATCTGTTCCCTGTTCCTTCTGGGTCA
  GGCTGTCACTTCCGGCTCTCAGCCCTATCTCCTGCAACCTCAGTGCCTCAGCCTGAGAGAGAGATGAGA
  TGCTCTTGGACTCCCCACTGCATCTGGGCTGCAGGGCCAGAGCTAGTCTGACCATTAGGTCAGTCTGCC
  TCCTGACAGTTTTTGCGTAGTCAAGCTCTAGGCGGTATGGGAATGGCTACCGGGACTCTAATGGGGTGA
  AAGAGAGGGGAGGCTTGCCTTTGAGAGCCTATATAGCCTTCCTGTGAGAGAGGATTAGATAGGGTTCCA
  ACTGGGCCTACAAGCTCAAGCCATACATAAAAGGACCTTGGGACATAAGAACCAATGATTGTGCATAAG
  TTCTAAATTAGAGACACATATAGTTTCTCTCTTTCAGCACCAGCTCTTGCCCCTATGCTGGGTACCAAG
  GGAGTTCTCCTAGCTGTGGCTTCTCTAGGTTCTAGGGGTGCAAGCCTCTGTGTGTTTGTTTGTGTGTGT
  CTGTGTGTGCGTATCCACACTAGGGGTGCAAGCCTCTGGGTGTGTGTGTGTGTGTGCGTGCGTGTGTGT
  GTGTGTGTCCGTGTGTGTGTGTGTGTGTGTCCACACTGGCCAGCCTCCCTACTTACCAAGGTTCTCCAC
  TGCTTACCTTTTCCAGTGGGACAGTACAGTGTGAGCCCCCGGGAAGTACTGCCTGACCTATCCTAAGCT
  TTTACACTTGGATTTTAGCCATCATATGTTGGCCAGGTCTCACTGCAGCCTGCCCGAGGCTAACTGGCT
  AGAGCCTCCAGGCCCTATGATGCTCCCTGCCCAGGCCATATCCTTTATTCCTGCTGAGCTTCCTGGCTG
  AATAGATGAAATGGGGTCAAGCCCAGGCAGCTCATTCACTACCTGTGATCCACCTCAGGGCACGGGCAA
  ACACATAGGCTTGCGTCTTAAAGCCAGCTCCTCTGCCAGACCCCGTTGTAATGTGCCACAACACCCTCA
  ATAGTCAGGGCAACTGGTGGAGCATGGAAGTCGAATTTCCTTTTCTGTTAGGAGCTACTCCTGGGAACC
  CCTCTCAGGGCTGCAGCTTACAGGTGGGCAGCTGTGATTGCACAACTTGAAGGGCCATCATTCACATCT
  ATTCAGTGGGAGTGGGGTCCCTGGGATTGGGCAGTGTGGTGGCCCTGTGTCTCCTCACCTCTGCTCCTG
  TCTTCATCACCTTCTCTCTGGAAGGGAAGAGGAGTTGGAAGGTCTCTGGTTTTCTTTTCTTTTTTTTTT
  TTTTTGCCAAAGGTTTACTTCCAGCATCTGAGCTCTGGCTCTCACCCCTGAAGCTCAGTTATAGTGCAC
  TGATGAACTGAGAGGATGCGTGTGGATGTGTGTGCATGCCTGAGTGCGTTTTTTGGGGAGGGGTGTTTA
  TTTTTAGTACCCCATTCTGGGGTTCTCTGATGCAGTGTGGATGTGAAGATATGGTACCTTCTCAAGTGT
  AGCTCTTTCAAATATAGTCAATGCTGGGAAATGTGATTGCAGTGATCTCTATCTCTCCACTTCTTTTGG
  GAAAGAGGAGCACAGGAGCGGAGGAAAAACTTGGCCACAGTCACACTTGGAAAGATAGTAGATTATTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_017902.3
• Summary: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.[UniProtKB/Swiss-Prot Function]
• Locus ID: 55662
• MW: 73.1