RGS5 (NM_003617) Human 3' UTR Clone

CAT#: SC222178

3' UTR clone of regulator of G-protein signaling 5 (RGS5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RGS5
• Synonyms: MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• ACCN: NM_003617
• Insert Size: 5074 bp
• Sequence Data:
  Insert Sequence

  >SC222178 3' UTR clone of NM_003617
  The sequence shown below is from the reference sequence of NM_003617. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCGCTTTGTGCGCTCTGAGTTTTATCAGGAGTTAATCAAGTAGTAATTTAGCCAGGCTATGAAATCATCC
  TGTGAGTTATTTCCTCCATAATAACCCTGCATTTCCCATTAATCTACATATCTTCCCACAGCAGCTTTGC
  TCAGTGATACCCACATGGGAAAAATCCCAGGGGATGTTGCTTACTCTTTTTGCCCACACTGCTTTGGATA
  CTTATCTACTGTCCGAAGGCCTTCTTTCCCCACTCAATTCTTCCTGCCCTGTTATTAATTAAGATATCTT
  CAGCTTGTAGTCAGACACAATCAGAATCACAGAAAAATCCTGCCTAAGGCAAAGAAATATAAGACAAGAC
  TATGATATCAATGAATGTGGGTTAAGTAATAGATTTCCAGCTAAATTGGTCTAAAAAAGAATATTAAGTG
  TGGACAGACCTATTTCAAAGGAGCTTAATTGATCTCACTTGTTTTAGTTCTGATCCAGGGAGATCACCCC
  TCTAATTATTTCTGAACTTGGTTAATAAAAGTTTATAAGATTTTTATGAAGCAGCCACTGTATGATATTT
  TAAGCAAATATGTTATTTAAAATATTGATCCTTCCCTTGGACCACCTTCATGTTAGTTGGGTATTATAAA
  TAAGAGATACAACCATGAATATATTATGTTTATACAAAATCAATCTGAACACAATTCATAAAGATTTCTC
  TTTTATACCTTCCTCACTGGCCCCCTCCACCTGCCCATAGTCACCAAATTCTGTTTTAAATCAATGACCT
  AAGATCAACAATGAAGTATTTTATAAATGTATTTATGCTGCTAGACTGTGGGTCAAATGTTTCCATTTTC
  AAATTATTTAGAATTCTTATGAGTTTAAAATTTGTAAATTTCTAAATCCAATCATGTAAAATGAAACTGT
  TGCTCCATTGGAGTAGTCTCCCACCTAAATATCAAGATGGCTATATGCTAAAAAGAGAAAATATGGTCAA
  GTCTAAAATGGCTAATTGTCCTATGATGCTATTATCATAGACTAATGACATTTATCTTCAAAACACCAAA
  TTGTCTTTAGAAAAATTAATGTGATTACAGGTAGAGGCCTTCTAGGTGAGACACTTTTAAGGTACACTGC
  ATTTTGCAAAAAAAAAAAAAAAAAGTAATCTTTTAGCAACCCCAGTATTCCTTCACTATTTCGCTTCCTG
  CATTAGCAAATTTTACTTACAGTCAAAAGTGCAGATTTATACTCCTGACGTGTCTCATTCACAGCTAAAT
  AATAGGCCATAGGACTTTTGGTAGGTTTAAACTTTTAATTCTGTATTTCATGATTATAAGTCTTGCTAGA
  ATTTTTTCTAATCTTTAGTAGATTTGATTAAATAATGATTCACAGAATTTAGTAACAGAATCAAACTAAG
  CCATGTATGAGGGTAATCGAGATGAGGATATTAACTCAAAAGAAATAGGGTGATTTTTAAAGGATTAATA
  AAATTCTGAAATGTTAAGTAGAAGATTACATTGTCTAGTCTTGTATTTCCTCCTTCTGTTGCTCTCTTTC
  ATTCACACACTCTCAGTTTCTCATATTTGTAGCTCATTTATTTGGTTATTTCCTAAGAATATTGAAAGTG
  AAGCAACTATGTGACTGTATTCTTCAGGTAAACACTGACTGCGCTTGTTGGATTTTCCCTATTTTTGTGA
  CTTCAAGAATAATATGCCCTGCTGAATACATGCCATTTCACATTCTGAAACTGGGTAGAGTGGTTGGGTG
  TTCTGCCAACAATTGCTAGTGGTGTGAATTCATTCATATTTGCCAGTATTGCTCACTTCAAAGAAACTCC
  TTCATCAAGCAGTCCAGAGCTAGGCCAGATCAATGCTACAATCATGAAGTTCTCATTGCATGCAATTGTG
  TAGGATTGACAAGGAACTCAGATAAAAATTTCCAGGGTGCACTTCCAGAACCAGCTTCAACATATGTCTA
  CATTGCCCCCAAGTTAATAAAGTGCCAACCCTTTACTCTCTCATACAGCCAGAAATGTTAGAAATCCAAA
  ATCTTGGTGCATTATTTTTTCATAAACGCTAAAACATTTGAAGAAACAATTTAATTATTTAAAATTCAAG
  TATTTTATTCACATTATTTGCAATATCCAAATGTTTAAAAATTCCCAGATAATTAACTAGCTATTACAGA
  TCTCACCTAGAGGGTTGATGTTATGAAGACTCCAGTGGACTGTACTCACAAATTGACTGGACACCCTATG
  AAAGTGGGTAGACCTCTCAGCGGAAAATAAGAAGGGCTTTTACCTACAGGGCAGGACAGGGTCCCATGAG
  AGCAGTTCTGTGGAGATATAAAAAGAATGGAAGAAGGAATGCCTTATAGTGATATTGTGACATTATATCT
  ATATATCTACATATATCTATCTATCTATATCTACATCTATATAATCTTACATTTAAAATTGTATTCCTAC
  ACATATTAGAAACTCTTCTAATAAATGAAGTAAAAAAATTAAAAAGAATACAAATATTCCAGCCCCAAAT
  GAGAAATCAAACATATTAAAATTGTTCAAGAAAATTTCTTTGAACACTTCTGAAAGTTTTTGGAAACTTA
  GAAAAGAGGGAAAAAAATCCAGTGTTACTAGTAATTTCCATGGTAATACAGATAAAATACATTCTTTTAA
  TTCTGGGAAATTAGAAAAAGTGGGGTGATCTTTCCAGGAAAAACATGTGTAACATCTGCTTATCACTCCA
  GCTCCCTCCTCCTCCTCCTCTCCACGTTCCCTTGAGTAAATGTCTGGGAAAGCATGAAGCTTGATGCAAG
  AACCCTGTTGTACTGGCGTTTTCCTCCCCTGTGAAAACGTAACTACTGTTGGGAGTGAATTGAGGATGTA
  GAAAGGTGGTGGAACCAAATTGTGGTCAATGGAAATAGGAGAATATGGTTCTCACTCTTGAGAAAAAAAC
  CTAAGATTAGCCCAGGTAGTTGCCTGTAACTTCAGTTTTTCTGCCTGGGTTTGATATAGTTTAGGGTTGG
  GGTTAGATTAAGATCTAAATTACATCAGGACAAAGAGACAGACTATTAACTCCACAGTTAATTAAGGACG
  TATGTTCCATGTTTATTTGTTAAAGCAGTGTGAATAGCCTTCAAGCATGTGAATAATCTTCCATCTTCCC
  CGCCACACATACACACACACACTTTTTGTTTCTTTCAGGTAGACACCTTTTAAAATGCAGAACTAACTGA
  GGCATTTCAGTAACTTTGCTTTCAAATCAATAAAGTCAAATGTATGGAAACATTTTGTGCCCTACTCTCC
  ATACCCCGTGTACTCAAATTCTCTACTGTATGAATTATGCTTTAAGTAGAATTCAGTGCCAAGGAGAACT
  TGGTGAAATAAATTATTTTAATTTTTTTTTTATCCTTTACAAAGCCATGGATTTTATTTGGTTGATGTGT
  GCTCTGTACACAAGCCATTTCAATAGGATGGAGCTGTTAATTATTTTCCAAAGAGTAATAGACATGCAAA
  AGTTTCAATAAAAACTGGGCCATTAACAAATAAATTAATAAACTAATAAGCATTCCCTTCTAGGTTTTTG
  CCAAACTGCCTATCCAATAACAAATTTGAGAATCGTTGAAAAAGCTAGTTATATTTCAGAGAAATGATTT
  TCATTATTGAAACTGTTCTCCCTAGCAGGCCATTTTCCCTTTTTCCTGGGAGTTTAGCAAGTTTAGGAGA
  GAATAGTCATGAAAAGAAAGGGAAGAAAGGGGAGAAGGGAAGAGGTTAAAAAGTAAGTGCTCAGACCTAT
  GAACGTAATCCCTTTGCTAGAAATATTTAAGAGCAGCTCAGCTTGGTTGAAACTGAGTTTTGTCATCTTC
  CATATTTGCAGGAAGGTATTTTCTGACTTGCAATGCAGCTAGATGTAAAATTTTATTTTATCATCCTAGA
  AAGCCTTGACTAGAAAAATGAATAAATATTGAGGGTTTCCTGTCCATATCTGGCTTGCATGTGCCAGAAA
  GCAGAGAATAGAAAATGTAATCTCCAACATCCAAGCATCGAAACCCAAGGGGTAGGCAATTCTATGTAGG
  TTTTGGACATGAAGTTTGGTGCATCTTGGTTTATGCTGGCTCAACTGCTATTAAACCTCTCTGGCTTATA
  GTCTCTTCATTCTATTAGACAAGCACGTATCGAACACTTGCTTCGCACAAGGCTCTTTAGTTAACAATTT
  AGCAGCTACTGTTTGTGTTAAACACACTTTTCACCAAATAGGTTCTGAGGCAAACGAGAGCAATGACTAT
  TTAAAGAAAGGCTTTCCCAGCATCACTTACACATCCCAAAACTAAAAAGATCAACTCTTCCAACTGAGAA
  AAGACTCCTGGCTTTGAATGGAAACTTACAGCAGAGAGTCACAGGCCACGGCAACAACAACGACAACAAC
  AAACATTTGGAATATTATTCTCAACTCACGTTTTAATAATACATCTTATTATTTTTCTAGTAGAGAAACT
  ACAAATCAGCCTCTTCAACATTTATATACAGTTTAATAAGCCTCTTGCAAGTTACTTGTTCTCTCACCTG
  AGGTATTTTTTTCCTCCCCACCTTGCCCCTGTTCCTCCCTTCCTCTTCTCCCTTTGCAAGAGGAAATATT
  TAACATATTTGGGTCCAACTTCAATAATGTAATAATTAATACATTAAAAGCATTTAACTTCCTTTCTAGA
  AAAATGCACAGGCTAAGGCATAGACAAAACAAAGAGAAATGCTGAGAAATTTGCCACTGGAGACAAGCAA
  TCTGAATAAATATTTGCCAAAAGTTCTTTTTATGTCATATAGTGTCAGGATTTGAAGGAGCTATTTTTTT
  TTAATGTTGCAACTAGCAACTCATCTTCGGAAGACACAGCCAGGAGAATGAAGTAGAAGTGAAAGGTTTA
  TAAATCCATTTGTAAGCATTTATCCCATATATTTTAAATTCAAGAAAAATTGTGTTTATCTTTAGAATTT
  TGTATTCAATACTTTATGTACTATGTGACTCATGCTTCTGGATAAATAAAGCACCAAATATGTATCTGTA
  ACCACAATCACACATATTATATTAAATATATATC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003617.2
• Summary: This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Dec 2011]
• Locus ID: 8490