Frizzled 5 (FZD5) (NM_003468) Human 3' UTR Clone

CAT#: SC221829

3' UTR clone of frizzled homolog 5 (Drosophila) (FZD5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FZD5
• Synonyms: C2orf31; HFZ5
• ACCN: NM_003468
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221829 3’UTR clone of NM_003468
  The sequence shown below is from the reference sequence of NM_003468. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CACAAGCAGGTGTCCCTGTCGCACGTGTAGGAGGCTGCCGCCGAGGGACTCGGCCGGAGAGCTGAGGGG
  AGGGGGGCGTTTTGTTTGGTAGTTTTGCCAAGGTCACTTCCGTTTACCTTCATGGTGCTGTTGCCCCCT
  CCCGCGGCGACTTGGAGAGAGGGAAGAGGGGCGTTTTCGAGGAAGAACCTGTCCCAGGTCTTCTCCAAG
  GGGCCCAGCTCACGTGTATTCTATTTTGCGTTTCTTACTGCCTTCTTTATGGGAACCCTCTTTTTAATT
  TATATGTATTTTTCTTAATTTGTAACTTTGTTGCATTTTGGCAACAATTTACCTTTGCTTTGGGGGCTT
  TACAATCCTAAGGTTGGCGTTGTAATGAAGTTCCACTTGGTTCAGGTTTCTTTGAACTGTGTGGTCTCA
  ATTGGGAAAATATATTTCCTATACGTGTGTCTTTAAAAAAAAATGTGAACAGTGAACGTTTCGGTTGCT
  GTGACTGGGAAGTTGTTGGGTGTGCTTTTTCAGCCAGCTTCTCCTTCCACTGCTTAAAGTGTCCATGAT
  TCTTTAAGGTGAGCTGCAGTTTATAGCCCCAGGTCATACCTAGGAGGGGAGCATAATGAGCTCAGGGCC
  TCCCCAAAGTGACAAGGTTAGGGAGTGCTTAGCGGTTTTGTGTTCAGCCTTAGCTTTGTTTATAGAGGG
  AGGTTCAGTTTCTTTTCTGTAGTGCTTGTAATAATTCTCACTCCTAACAGCACCATCGTTGTGTCTTGA
  ATAAGTTAGAGGTAGCATTATAGAGGATCTGGCATAAATATTTGCAGTAGTGAGAGCCTAAGCGATGGT
  GATTGGTGGAGCTTGAATTTTAGGCTGGTGAGATGGCAGCTTTGTGCCTGAGAGGTAGTGGGTGGTTCT
  TAAGCTTCAGTGATCCCCTTTTTTTTTTTTTTTTTTTTTTTTTAAGGAACTTGTGTTATAATTTTGGTA
  AAAGTATAAACCCACTCCCTCTGGACAATACTTAGCGACAGTTGCTAAAGGGGGCTCCTTTTTAAATGT
  AAGGACTGAAATGGATATACTTCTAATAAGTAAATTTCCAACACTTATTTGCTCCACCCCCTCCCCCCT
  CCCCCCTCCCCCTTTATCATGTTAAACAGCCTTTTTGCTTTTCTTATTCCTCCTCTCCTGGAGAGCTGT
  GATTAGAAACCACACCCACCCTTGAATGAAGTGCTTGAACTGGGGGAGGGAGGCTGGCTACCTGTGAAC
  AAACATTGGCCCAAATAAGGGAAAATAAGTGTTCCTGGACTTTGGACTAGTTTATAGCCAGATATTCCA
  AGAGCAGCAAGACGTTGCTCTCTGCCGTCTCTGAAAACAAAAGAGATGCATAACATGCTTGCACAACCT
  TTTAAAATATAGATCAGTATAGTGCTACCTCTATAGTTTTCTTCCTCTTCTGAGAAAGCCTGTATATTG
  ATGATCACACACACACACACTTTGCAATTAGAGAATTTGGTTTGCTTTACTAATCTGTTTAACTATTCC
  TTCATTCATTATGAACGCTTATATTGATGAACATACACACAGAGGTTTCTTTGCTATTAGAAAATTCTG
  TTTGCTTTCCTAATCTGTTTAAGCATTCATTCATGAAGAGTGTGGGGCCATTACTGGGGAAGGGGGGTG
  ACAGTGCCTCAGCCAGCAAAATACCAATGACCAGGATTGGGGACTAAATTTAGGAAGCTAAAATGGCCA
  GAGCAATTAACATTTGAGAAAATCCTGTCTAGGAAAACAACTTGAGTGTAGGCATTTGTAATTCACTTA
  TACCAAAGTTGGAAAAGTAAAATTTAAGCCTAGGACAATTTTTACTTCATGGATGTTAAATAGACAAAT
  GCATAGTTCCCAGGGGGAATTTAAACACTTTACTGGTGGGAAGAAACCTAGTATTAAAGTTGTAAGGAC
  TCTCAAAAACTTCACATTTATTAAAATGCACTGCTCTTACCCAATTTATCCTCTGAATTAAAATTTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003468.4
• Summary: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008]
• Locus ID: 7855
• MW: 75.6