RUNX1 (NM_001001890) Human 3' UTR Clone

CAT#: SC221763

3' UTR clone of runt-related transcription factor 1 (RUNX1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RUNX1
• Synonyms: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha
• ACCN: NM_001001890
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221763 3’UTR clone of NM_001001890
  The sequence shown below is from the reference sequence of NM_001001890. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGGAGGAGGCCGTGTGGAGGCCCTACTGAGGCGCCAGGCCTGGCCCGGCTGGGCCCCGCGGGCCGCCG
  CCTTCGCCTCCGGGCGCGCGGGCCTCCTGTTCGCGACAAGCCCGCCGGGATCCCGGGCCCTGGGCCCGG
  CCACCGTCCTGGGGCCGAGGGCGCCCGACGGCCAGGATCTCGCTGTAGGTCAGGCCCGCGCAGCCTCCT
  GCGCCCAGAAGCCCACGCCGCCGCCGTCTGCTGGCGCCCCGGCCCTCGCGGAGGTGTCCGAGGCGACGC
  ACCTCGAGGGTGTCCGCCGGCCCCAGCACCCAGGGGACGCGCTGGAAAGCAAACAGGAAGATTCCCGGA
  GGGAAACTGTGAATGCTTCTGATTTAGCAATGCTGTGAATAAAAAGAAAGATTTTATACCCTTGACTTA
  ACTTTTTAACCAAGTTGTTTATTCCAAAGAGTGTGGAATTTTGGTTGGGGTGGGGGGAGAGGAGGGATG
  CAACTCGCCCTGTTTGGCATCTAATTCTTATTTTTAATTTTTCCGCACCTTATCAATTGCAAAATGCGT
  ATTTGCATTTGGGTGGTTTTTATTTTTATATACGTTTATATAAATATATATAAATTGAGCTTGCTTCTT
  TCTTGCTTTGACCATGGAAAGAAATATGATTCCCTTTTCTTTAAGTTTTATTTAACTTTTCTTTTGGAC
  TTTTGGGTAGTTGTTTTTTTTTGTTTTGTTTTGTTTTTTTGAGAAACAGCTACAGCTTTGGGTCATTTT
  TAACTACTGTATTCCCACAAGGAATCCCCAGATATTTATGTATCTTGATGTTCAGACATTTATGTGTTG
  ATAATTTTTTAATTATTTAAATGTACTTATATTAAGAAAAATATCAAGTACTACATTTTCTTTTGTTCT
  TGATAGTAGCCAAAGTTAAATGTATCACATTGAAGAAGGCTAGAAAAAAAGAATGAGTAATGTGATCGC
  TTGGTTATCCAGAAGTATTGTTTACATTAAACTCCCTTTCATGTTAATCAAACAAGTGAGTAGCTCACG
  CAGCAACGTTTTTAATAGGATTTTTAGACACTGAGGGTCACTCCAAGGATCAGAAGTATGGAATTTTCT
  GCCAGGCTCAACAAGGGTCTCATATCTAACTTCCTCCTTAAAACAGAGAAGGTCAATCTAGTTCCAGAG
  GGTTGAGGCAGGTGCCAATAATTACATCTTTGGAGAGGATTTGATTTCTGCCCAGGGATTTGCTCACCC
  CAAGGTCATCTGATAATTTCACAGATGCTGTGTAACAGAACACAGCCAAAGTAAACTGTGTAGGGGAGC
  CACATTTACATAGGAACCAAATCAATGAATTTAGGGGTTACGATTATAGCAATTTAAGGGCCCACCAGA
  AGCAGGCCTCGAGGAGTCAATTTGCCTCTGTGTGCCTCAGTGGAGACAAGTGGGAAAACATGGTCCCAC
  CTGTGCGAGACCCCCTGTCCTGTGCTGCTCACTCAACAACATCTTTGTGTTGCTTTCACCAGGCTGAGA
  CCCTACCCTATGGGGTATATGGGCTTTTACCTGTGCACCAGTGTGACAGGAAAGATTCATGTCACTACT
  GTCCGTGGCTACAATTCAAAGGTATCCAATGTCGCTGTAAATTTTATGGCACTATTTTTATTGGAGGAT
  TTGGTCAGAATGCAGTTGTTGTACAACTCATAAATACTAACTGCTGATTTTGACACATGTGTGCTCCAA
  ATGATCTGGTGGTTATTTAACGTACCTCTTAAAATTCGTTGAAACGATTTCAGGTCAACTCTGAAGAGT
  ATTTGAAAGCAGGACTTCAGAACAGTGTTTGATTTTTATTTTATAAATTTAAGCATTCAAATTAGGCAA
  ATCTTTGGCTGCAGGCAGCAAAAACAGCTGGACTTATTTAAAACAACTTGTTTTTGAGTTTTCTTATAT
  ATATATTGATTATTTGTTTTACACACATGCAGTAGCACTTTGGTAAGAGTTAAAGAGTAAAGCAGCTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001001890.3
• Summary: Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 861
• MW: 77.1