ADAR1 (ADAR) (NM_001111) Human 3' UTR Clone

CAT#: SC219612

3' UTR clone of adenosine deaminase RNA-specific (ADAR) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADAR
• Synonyms: ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
• ACCN: NM_001111
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC219612 3’UTR clone of NM_001111
  The sequence shown below is from the reference sequence of NM_001111. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAAAAGAACTTTTATCTCTGCCCAGTATAGTATGCTCCAGTGACAGATGGATTAGGGTGTGTCATACTA
  GGGTGTGAGAGAGGTAGGTCGTAGCATTCCTCATCACATGGTCAGGGGATTTTTTTTTCTCCTTTTTTT
  TTCTTTTTAAGCCATAATTGGTGATACTGAAAACTTTGGGTTCCCATTTATCCTGCTTTCTTTGGGATT
  GCTAGGCAAGGTCTGGCCAGGCCCCCCTTTTTTCCCCCAAGTGAAGAGGCAGAAACCTAAGAAGTTATC
  TTTTCTTTCTACCCAAAGCATACATAGTCACTGAGCACCTGCGGTCCATTTCCTCTTAAAAGTTTTGTT
  TTGATTTGTTTCCATTTCCTTTCCCTTTGTGTTTGCTACACTGACCTCTTGCGGTCTTGATTAGGTTTC
  AGTCAACTCTGGATCATGTCAGGGACTGATAATTTCATTTGTGGATTACGCAGACCCCTCTACTTCCCC
  TCTTTCCCTTCTGAGATTCTTTCCTTGTGATCTGAATGTCTCCTTTTCCCCCTCAGAGGGCAAAGAGGT
  GAACATAAAGGATTTGGTGAAACATTTGTAAGGGTAGGAGTTGAAAACTGCAGTTCCCAGTGCCACGGA
  AGTGTGATTGGAGCCTGCAGATAATGCCCAGCCATCCTCCCATCCTGCACTTTAGCCAGCTGCAGGGCG
  GGCAAGGCAAGGAAAGCTGCTTCCCTGGAAGTGTATCACTTTCTCCGGCAGCTGGGAAGTCTAGAACCA
  GCCAGACTGGGTTAAGGGAGCTGCTCAAGCAATAGCAGAGGTTTCACCCGGCAGGATGACACAGACCAC
  TTCCCAGGGAGCACGGGCATGCCTTGGAATATTGCCAAGCTTCCAGCTGCCTCTTCTCCTAAAGCATTC
  CTAGGAATATTTTCCCCGCCAATGCTGGGCGTACACCCTAGCCAACGGGACAAATCCTAGAGGGTATAA
  AATCATCTCTGCTCAGATAATCATGACTTAGCAAGAATAAGGGCAAAAAATCCTGTTGGCTTAACGTCA
  CTGTTCCACCCGGTGTAATATCTCTCATGACAGTGACACCAAGGGAAGTTGACTAAGTCACATGTAAAT
  TAGGAGTGTTTTAAAGAATGCCATAGATGTTGATTCTTAACTGCTACAGATAACCTGTAATTGAGCAGA
  TTTAAAATTCAGGCATACTTTTCCATTTATCCAAGTGCTTTCATTTTTCCAGATGGCTTCAGAAGTAGG
  CTCGTGGGCAGGGCGCAGACCTGATCTTTATAGGGTTGACATAGAAAGCAGTAGTTGTGGGTGAAAGGG
  CAGGTTGTCTTCAAACTCTGTGAGGTAGAATCCTTTGTCTATACCTCCATGAACATTGACTCGTGTGTT
  CAGAGCCTTTGGCCTCTCTGTGGAGTCTGGCTCTCTGGCTCCTGTGCATTCTTTGAATAGTCACTCGTA
  AAAACTGTCAGTGCTTGAAACTGTTTCCTTTACTCATGTTGAAGGGACTTTGTTGGCTTTTAGAGTGTT
  GGTCATGACTCCAAGAGCAGAGCAGGGAAGAGCCCAAGCATAGACTTGGTGCCGTGGTGATGGCTGCAG
  TCCAGTTTTGTGATGCTGCTTTTACGTGTCCCTCGATAACAGTCAGCTAGACACACTCAGGAGGACTAC
  TGAGGCTCTGCGACCTTCAGGAGCTGAGCCTGCCTCTCTCCTTTAGATGACAGACCTTCATCTGGGAAC
  GTGCTGAGCCAGCACCCTCAGATGATTTCCCTCCAAACTGCTGACTAGGTCATCCTCTGTCTGGTAGAG
  ACATTCACATCTTTGCTTTTATTCTATGCTCTCTGTACTTTTGACCAAAAATTGACCAAAGTAAGAAAA
  TGCAAGTTCTAAAAATAGACTAAGGATGCCTTTGCAGAACACCAAAGCATCCCAAGGAACTGGTAGGGA
  AGTGGCGCCTGTCTCCTGGAGTGGAAGAGGCCTGCTCCCTGGCTCTGGGTCTGCTGGGGGCACAGTAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001111.5
• Summary: This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
• Locus ID: 103
• MW: 74.8