Alanyl tRNA synthetase 2 (AARS2) (NM_020745) Human 3' UTR Clone

CAT#: SC216616

3' UTR clone of alanyl-tRNA synthetase 2 mitochondrial (putative) (AARS2) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AARS2
• Synonyms: AARSL; COXPD8; LKENP; MT-ALARS; MTALARS
• ACCN: NM_020745
• Insert Size: 1846 bp
• Sequence Data:
  Insert Sequence

  >SC216616 3’UTR clone of NM_020745
  The sequence shown below is from the reference sequence of NM_020745. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCCAAACCTATGCCCTCAGCCAGCTCTGACCCAGGTCCGCCCAGGGACCCACATGGACTAAAGGCAAT
  GCCAGGAGCCCTGAAGGAGCCAGCAGAACCTTCCCACATGCTGAAGCGAGGACTAGAGGCTGGAGGCCA
  AGCAGCTGAGCCAAAGAAGATCACCTGGGCCAGGGCCTACGTGACACAAGGAGACATGGGCTAGGGAAG
  GCAGAACTGGGCCCAAAGACACACACCCCCTTGGTGAAAGAGAAGACATGGACAATTATGGCTGTGACT
  GCACATTAGAAATTATTTCACTGGCCGGGTGTGGTGGTTCACACCTGTAATCCAAGCACTTTGGGAGGC
  CGAGGCAGGTAGATCATGAGGTCAGGAGATCAAAACCATCCTGGCTAACATGGTGAAACCCCATCTCTA
  CTAAAAATACAAAAAATTAGCCGGGTGTGGTGGCACACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG
  GCAGAAGAATCGCTTGAACCTGGGAGGTGGAGGTTGCAGTGGGCCAAGATCGCACCACTGCACTCCAGC
  CTGGGTGACAGAGCGAGACTCTGTCTCAAATAATTATTTCACAGAGGATAGCAAGCTCCAGAAGGTTCT
  ATTACCTTTAAAAAAGAGTAGTTTTGCCCCTCCCAGCAACCTAAGACCAGTCTGAACTCACACTGTCTG
  AAAGGCCACCCAGGATCCAAATCGGGCCATCTTCATTGCCATGAGTGTTCGGACAGGCTCTGCGTGCCA
  GGCAACAGTGGGCAGACAAAGGGCTCCCTGGCTACTGGGAAGATACAGAACCATCCTGTGAAAATCATT
  TCAGCACTGGAGACCTTCTTTGCTATTACTTCTTTCATTAACAATGACCTCTAATTTAAATTGGGCCCT
  GCTGGTTTGTTGATGAATTGAGCAACTGAGAACGTGGCGGGCACTAAACTGACAGTTCCTTCCTTCATG
  GAGCCTGCTTCATGTGGAGGAAACAGGAAATAAAGCAAAAGAAGAGGGTCAGGCTTGGTGGCTCATGCC
  TATAATCCTGGCATTTTAGGAGGCTGAGGTTGGAGAATCGCTTGAGGCCAGAAGTGCAAGACCAGCCTG
  CGCAAACAGCAAGACCCTGTCTACAAAAAAAAAAAAAAGTAATCTTTATGCCTCAGCCTCCCAGGTAGC
  TGAGACTACCCACACCTTGGTCCCAGCTACTTGGGAGGCTGAGGTGGGAAAATCACTTTGCCCAGGAAT
  TCAAGGCCGCAGTGAGCTATGATTGCACCACTGCACTCCAGGCAACAGAGTGAGACCCTGTCTTAAAAA
  AAAGAAGGGAGAAAGTGTCAGATGGTGATGAGGTCTGGGGGGGAAATAGAGAATGGGGATCAGGAGTGT
  GGATGGTGATATTCCCTCACCAAGAGGTGACATGTGAGCAGGGAGCTGGGAGGTGAGGGTGTGACCCGT
  GTGGAAATCAGGGAAAAGCATTCCAGCCTGAGGGAACAGCCAATGCAGAGGCCGTGAGGTGGCCAGTGC
  CACTGAGCAGTGAGCTGGGATAGGGGCAGGTGAGGAGGCTGGAGAGCGGGGTCAGACAAACAATATGCT
  TATTAAAACAAGTTGTACAGCACCTGCCTCAAGCCTGAGCTGAAGCTGAAAATTGGCACTTCAAAGCAG
  GAGGAAGCAGAGATGGAGGAAAGCCCTCCTTCCCTACCAGAATGAGTCCACCTCAGACAGCCAGACTTT
  GGACCTCTGCCTTTGTTCCTAGATCTCTGTTGCTAGATGAAGGAAAGGGCCTCAGGGGATCCATCTTCA
  AATCAAGGGCTCCCTGGCTACCAAATTCTGTCTAGTAAAAATTATTCGAGCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020745.4
• Summary: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8. [provided by RefSeq, Dec 2011]
• Locus ID: 57505
• MW: 67.8