SASS6 (NM_194292) Human 3' UTR Clone

CAT#: SC216472

3' UTR clone of spindle assembly 6 homolog (C. elegans) (SASS6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SASS6
• Synonyms: MCPH14; SAS-6; SAS6
• ACCN: NM_194292
• Insert Size: 1788 bp
• Sequence Data:
  Insert Sequence

  >SC216472 3’UTR clone of NM_194292
  The sequence shown below is from the reference sequence of NM_194292. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TATTTCCCTGGGCAGTTACCAAACAGTTAATTCTAGTGTCATGTTTTACTTTTTATTGGTATTTTAGAA
  ACTCAGGTGCTTAAAAAATATGTCAACACAAACCAGATCCTCAAATAGCAAGTTTCAAATTTTACTTGA
  GCTGTTAAAGACTGGATACTTTAAGTACTGCTTTTATGGCAGTTTATAATATAGGAACAAATTTGTACT
  TGGGGATTGGTAAAGATTGTGAGTAAATGACATTTTTTGGTTTGTGAACATGGCGTTTTATAGAATAAT
  ATGAATTTTATTAAGCTATTCTTTAGAAATTCAGTTCTAAGGAAAGAGTTGATCCTTATGAGGGAGATT
  CTGATCATATTAAAAATCTGGGTAAACTAAAACCTGCTCATAACCTCATAGTATGCAACCAAAGAATAC
  TAATTCATTTTATCACTTTCATTGGTAAAACTTTTAAATTCAACACCTGAAAATCATGAGGGAAAATGA
  TTACCTCTTGAGATATATTATTGATGACATTTAAAGGGAAAAGTCCTAAATTTACCTTAAATAACTGAA
  ATGAATTAAGCCTTCATAGCGTATTTTCCTTGAAGCATTTAAATTTGCTAGTGATGAATCAAATAAATT
  TGATATACTTAAAATAATTTGGACAGTTTTTATGTGACCAGATTAAGTGCTTTTGTCATTTGAGCTGAC
  AAGTTTTTAAAACTTCAGTGCTTCCTAAATGTTTATACTCATCTAGTTTTTCTCAATTTGTAGTTTTAT
  TATGCAGTTAAATCTGTATCAGTGGACTTTTAAAACTTGGTACTACTACTAAATCTACTATATCATGAA
  TATTCATTTATTTTTATAGTATCAGAACTTGGGTTGATTTCAATGTTAATAAACTAGGCAAAACAAATA
  ACTGTTAGAAGGTACAATTTTCTTCTCTTCCCTGTAACTGAAAGTTTTAAGAATTGTTCCTTGGGGAAA
  AATTTTTTTAAAATTCACCTAGAATCATAGGGCCTCATTTTGTTTTCTTATTGACAAACAATACCTGGT
  AGCATTCTCTAAAGAGTCTTCTAGATCAATTTTTATAAAGTACTAATGCTCATGTGATAATTTAAAATT
  ATTTTCAACTACTTTATTACACAGATGAAGAAACATTTCAAGATATTGATGTAATAACATTACTGAGTC
  CCATGGTAAGAAAGAATCTTTGCTAATTTTTTTTTTTTTTTTACGAAGCCAGTATCTTGAAACTATTGG
  CAATTGCAAGTGTCTTTAAACTAGGGATCTAATCTGAAAATTCAGTAACTAATATTAGGCAGTTGATAC
  TCCCCCACCTCCCCCATTTAGATACACTACTGTATTAGTGAGAATACTTGGTACAGTAACTTGAAAGCA
  CTTGCTATTCTGGAAAGAAGCCTCTTGGTGATTTTGGAAGGAGCTTTATTGTAAATACTATGGAAGGGG
  CTTTGTTGTAAATACTATGTAATATATATAAAATATTGTGACATGTTTTGGATGGGATGCATGACATAC
  AGGTAGCTCTCTTTTTTAAAATATAGGTATCTCTTTGCATATATATACACAAGGAAAGTTGAATTTTCA
  GGAGAGATCATTCTGATATCTTGCAAATTTGTATTAAATGTATACAGACTTCCAAGTGTGCTAATTCTT
  TACCTCCTAAACTTTTAGTTGCAATTGTTTTGCAGTGTAAAATTGTACATGTGTTAATAATCAAAAAGT
  GTTGCATTTAATGTTATTTCTGTAAACTTTGAGCTTTGTTAATAAACTATTATGCAGCATCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_194292.3
• Summary: The protein encoded by this gene is a central component of centrioles and is necessary for their duplication and function. Centrioles adopt a cartwheel-shaped structure, with the encoded protein forming the hub and spokes inside a microtubule cylinder. Defects in this gene are a cause of autosomal recessive primary microcephaly. [provided by RefSeq, Oct 2016]
• Locus ID: 163786
• MW: 70.4