Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human 3' UTR Clone

CAT#: SC215377

3' UTR clone of casein kinase 1 alpha 1 (CSNK1A1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CSNK1A1
• Synonyms: CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975
• ACCN: NM_001892
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC215377 3’UTR clone of NM_001892
  The sequence shown below is from the reference sequence of NM_001892. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAACCAAGAGTAACATGAAAGGTTTCTAAGCATGAATTGAGGAACAGAAGAAGCAGAGCAGATGATCG
  GAGCAGCATTTGTTTCTCCCCAAATCTAGAAATTTTAGTTCATATGTACACTAGCCAGTGGTTGTGGAC
  AACCATTTACTTGGTGTAAAGAACTTAATTTCAGTATAAACTGACTCTGGGCAGCATTGGTGATGCTGT
  ATCCTGAGTTGTAGCCTCTGTAATTGTGAATATTAACTGAGATAGTGAAACATGGTGTCCGGTTTTCTA
  TTGCATTTTTTCAAGTGGAAAAGTTAACTAAATGGTTGACACACAAAAATTGGTGGAGAAATTGTGCAT
  ATGCCAATTTTTTGTTAAAACCTTTTGTTTTGAACTATACTGCTTTGAGATCTCATTTCAGAAGAACGG
  CATGAACAGTCTTCAGCCACAGTTGTGATGGTTGTTAAATGCTCACAATTGTGCATTCTTAGGGTTTTT
  CCATCCCTGGGGTTTGCAAGTTGTTCACTTAAAACATTCTTAAAATGGTTGGCTTCTTGTCTGCAAGCC
  AGCTGATATGGTAGCAACCAAAGATTCCAGTGTTTGAGCATATGAAAGACTCTGCCTGCTTAATTGTGC
  TAGAAATAACAGCATCTAAAGTGAAGACTTAAGAAAAACTTAGTGACTACTAGATTATCCTTAGGACTC
  TGCATTAACTCTATAATGTTCTTGGTATTAAAAAAAAAGCATATTTGTCACAGAAATTTAGTTAACATC
  TTACAACTGAACATGTATGTATGTTGCTTAGATAAATGTAATCACTGTAAACATCTATATGATCTGGGA
  TTTTGTTTTTATTTTGAAATGGGAGCTTTTTTGTTTACAAGTTCATTAAAAACTAAAAACTGTTTCTGT
  AAGGAAATGAGATTTTTTTTAAACAACAAAAAATGCCTTGCTGACTCACTATTAAATAAAAATCTCCCC
  AATTTTTTGATAGACTACTTCAAGCCATTTGTTACATGGTATTCCTTTGCAAGTCAATTTAGGTTTCGT
  GTTATAACTTTTCCTCTTTTTTTAAGAAAAATGAAAAAAGTAATTCTTTTGTCTGAAGGGGAAAGGCAT
  TCTTTCATTTTTTTCTTTTTTTTTTTTTTTTTTTATGACTTGCAGGCACAATATCTAGTACTGCAACTG
  CCAGAACTTGGTATTGTAGCTGCTGCCCGCTGACTAGCAGCTGGACTGATTTTGAATAAAAATGAAAGC
  ATTAAAGGGTTTCCCTACAAAACATTTTTCTTTAAAATACTTTTGAAATGGCTATAAGCAGTTGACTTT
  CACCCTTGGAGAGCATCACACTGTGTGAGGTTCAGTGATTGTTGACCCTCCCCAGCCCCTCCTGCTTCT
  TTAAGTTATCTGTGTGCGTGCGCTTCCTCTCAATCTTCTTTGCACGCTCATTTCTTTTTCTCTGACCCA
  TGAGAAAGGAAAACTTACTGATGATAATTTTTAAATAGTGTAATTTATTCATTTATAGCATGTCAGGAT
  AAATTAAAAGAACATTTGTCTGGAAATGCTGCCGGGAGCCTATTGTGTAAATGTAGGTATTTTGTAAAA
  TAACCTTGAAATTGTAAATTGACACGTGTTTGGTCAGATTGTGTCAAGTTTAATTTGTTTTGTTTTCTT
  TTTTCTTTTTTTTATTTGAAAACTACTTTAGCAATAATTAATTCCATGATTATCACATTCTGCCATTAA
  GGGATATTAGTACCGTAATACTGAAGAAATTTTATTAAGTCTGAACTTCTGGGGTAGGCAGCTTCTTTG
  TTTCTTTTCTATCCACCCTTGTCGGTTGAGGTATTTGTTTCTTGACTAATAAACCCTTTGATACTTTTA
  GCCAGAAATCAGTCTCATAAAGCTATTTTTGAGTATAGTTTGTGTAAAATAAAAATGTTTAGCTTTGGT
  AATAACTTCCAAGCTGAACTCCCTCTAGCAAGATATTTTTCAGTGCTTTTATTTACTATGCACTTAGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001892.6
• Summary: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).[UniProtKB/Swiss-Prot Function]
• Locus ID: 1452
• MW: 77.1