GPR56 (ADGRG1) (NM_001145770) Human 3' UTR Clone

CAT#: SC214727

3' UTR clone of G protein-coupled receptor 56 (GPR56) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADGRG1
• Synonyms: BFPP; BPPR; GPR56; TM7LN4; TM7XN1
• ACCN: NM_001145770
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC214727 3’UTR clone of NM_001145770
  The sequence shown below is from the reference sequence of NM_001145770. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCGGGCAGCACCTCGTCCAGCCGCATCTAGGCCTCCAGCCCACCTGCCCATGTGATGAAGCAGAGATTC
  GGCCTCGTCGCACACTGCCTGTGGCCCCCGAGCCCGGCCCAGCCCCAGGCCAGTCAGCCGCAGACTTTG
  GAAAGCCCAACGACCATGGAGAGATGGGCCGTTGCCATGGTGGACGGACTCCCGGGCTGGGCTTTTGAA
  TTGGCCTTGGGGACTACTCGGCTCTCACTCAGCTCCCACGGGACTCAGAAGTGCGCCGCCATGCTGCCT
  AGGGTACTGTCCCCACATCTGTCCCAACCCAGCTGGAGGCCTGGTCTCTCCTTACAACCCCTGGGCCCA
  GCCCTCATTGCTGGGGGCCAGGCCTTGGATCTTGAGGGTCTGGCACATCCTTAATCCTGTGCCCCTGCC
  TGGGACAGAAATGTGGCTCCAGTTGCTCTGTCTCTCGTGGTCACCCTGAGGGCACTCTGCATCCTCTGT
  CATTTTAACCTCAGGTGGCACCCAGGGCGAATGGGGCCCAGGGCAGACCTTCAGGGCCAGAGCCCTGGC
  GGAGGAGAGGCCCTTTGCCAGGAGCACAGCAGCAGCTCGCCTACCTCTGAGCCCAGGCCCCCTCCCTCC
  CTCAGCCCCCCAGTCCTCCCTCCATCTTCCCTGGGGTTCTCCTCCTCTCCCAGGGCCTCCTTGCTCCTT
  CGTTCACAGCTGGGGGTCCCCGATTCCAATGCTGTTTTTTGGGGAGTGGTTTCCAGGAGCTGCCTGGTG
  TCTGCTGTAAATGTTTGTCTACTGCACAAGCCTCGGCCTGCCCCTGAGCCAGGCTCGGTACCGATGCGT
  GGGCTGGGCTAGGTCCCTCTGTCCATCTGGGCCTTTGTATGAGCTGCATTGCCCTTGCTCACCCTGACC
  AAGCACACGCCTCAGAGGGGCCCTCAGCCTCTCCTGAAGCCCTCTTGTGGCAAGAACTGTGGACCATGC
  CAGTCCCGTCTGGTTTCCATCCCACCACTCCAAGGACTGAGACTGACCTCCTCTGGTGACACTGGCCTA
  GGGCCTGACACTCTCCTAAGAGGTTCTCTCCAAGCCCCCAAATAGCTCCAGGCGCCCTCGGCCGCCCAT
  CATGGTTAATTCTGTCCAACAAACACACACGGGTAGATTGCTGGCCTGTTGTAGGTGGTAGGGACACAG
  ATGACCGACCTGGTCACTCCTCCTGCCAACATTCAGTCTGGTATGTGAGGCGTGCGTGAAGCAAGAACT
  CCTGGAGCTACAGGGACAGGGAGCCATCATTCCTGCCTGGGAATCCTGGAAGACTTCCTGCAGGAGTCA
  GCGTTCAATCTTGACCTTGAAGATGGGAAGGATGTTCTTTTTACGTACCAATTCTTTTGTCTTTTGATA
  TTAAAAAGAAGTACATGTTCATTGTAGAGAATTTGGAAACTGTAGAAGAGAATCAAGAAGAAAAATAAA
  AATCAGCTGTTGTAATCACCTAGCAAACTGGCGTAAGCATTTTGGGTCATTTCCTTCTGAGCTTTTTCC
  TGTGCTTCTATTGAAAGAGTCACTGATACTAATACAAATACCTTGGGGCCAATCAGGAAACATGAAAGA
  GAGAAGAGGGCTGTGTGTGAAGAAAAAAAAAAAATCGACTGCTGGAAACCTCATCAACCAGGGCACAGG
  GGAAGATAAATGGCAACTTGGTGTCTGTGCTGGGGAGGCAATGGGGAGTGGTGGGGCCTGTGGCGAACT
  GGAGACTCCCAACCCCATGCTGAGAGCGTGGCTGCTCATCAGCTCCAGCTGGTCACTACCTTGCAGGGA
  TGAGGGCCCATGTAGCCAGACCACCTGCCTTTTATGAGAAATGGGAAATCCAGACTGTGGTTTGACATC
  CCCTGATTTTAAAATTTTGGCGAATCCAAAACATTTTCTGGCCAAAGAGATTACAGTCAAAAGCTGCCT
  GGTCCTGGGATTTATGCAACCAGTTTACTGATCACTCTGCAGCAGGTGGGCTGCAAGAATTAAATCAC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145770.3
• Summary: This gene encodes a member of the G protein-coupled receptor family and regulates brain cortical patterning. The encoded protein binds specifically to transglutaminase 2, a component of tissue and tumor stroma implicated as an inhibitor of tumor progression. Mutations in this gene are associated with a brain malformation known as bilateral frontoparietal polymicrogyria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
• Locus ID: 9289
• MW: 72