Rab5 (RAB5A) (NM_004162) Human 3' UTR Clone

CAT#: SC214172

3' UTR clone of RAB5A member RAS oncogene family (RAB5A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAB5A
• Synonyms: RAB5
• ACCN: NM_004162
• Insert Size: 1382 bp
• Sequence Data:
  Insert Sequence

  >SC214172 3’UTR clone of NM_004162
  The sequence shown below is from the reference sequence of NM_004162. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCAACCAGGAATCAGTGTTGTAGTAACTAAACCTCTAGTTTGAACTAGCTGGAATAGTCTTCTGCTTCC
  TAAATGTTAATAACAATGGAATTGGAGCATTTAACCAGCCCAGTATGACTTCCAAAAGAAGAGACTTAT
  GATAGAGTCAAGTTTCTAATACAGAATTATTTTAAGTGTTTTGAACTTAATTTTTAATAACATGCATGG
  GTCCCTCTCACTAATGTTTCAACAATAGGGAAAAATGAGAACTATGTGGACACTTGTTTCATTGGAAGG
  TTAGGGGGAATAATTTCTCATCACTAGGAATATAGACAAATGACTGTCTGGGCCCACACAGTTAACCAG
  CCCATTTCTCCACACTGGTACAGTAGTCACCTGTGAAAAAAAAATTGGAACTTACTAATTTGGGCTTTT
  CAAAAACATTCTTTGTTTAGAAGGAGATTCTAAAGTTATTTATGATGCTTAGCCATAGTATTCAGGCAA
  ATGTTCATTTCTCCTGGTACCTGTATTTAAAATGTACATTCCACATTTTAATAAATTAACCACAAGAAA
  ATAATCCCACATATACAAGGTCAGGGGTGGGGAAGAGTATTAATGGTATCTTAATTATACCCAGTCTGG
  TTTTTTTTTTTTAAATGGGGTAAAAATCAAATGCAACCCCATCTTGTTTTAGGAATTTTGAGAACTAAT
  AAATGCACCTTAATGGTCAGTGTTCCTTTCAAACATGTGAGTTCTTTAACAAAAATGAAATAAACCAGG
  TGTCTGTGATTTCTAATTAATCACCGCTGGCCATTACACAGGTTTTGTTGTTTGGGGTGGGGAGGGGGC
  TTTTGTTCCCTTTTGACATAATATAGTCAATGCACTAACAATTATGTATATTCAAACTTGATTATTTTA
  AATTCGATCTTCAGCTGTACTGTAAATAGGGTACTGCATTGTAGTCTCCATATCTGTATTACTTTTCTG
  TAATATTTAAGAGTTGCTTAAAAGCATACAAAATGTACTGTTACTAAAACAGCTAATTATTTCTCTCTC
  CCCCTTTGACAGGAAGGGGCTTCAGTTGTTCCTCCATGGCTAGAACCATAATAAACAATGTACCCGTAA
  TTTGTAACATAAAGTATTGCAATATGTTAGTAACAATCTTGCAGCCTTCCTTTCCAAAGTTCATTTTAT
  TTTGATCAGTTCAGTATATTGCACTAATTATTTTAGGTATTTTCATTATATGAAAGCTACCATGTGTCA
  GAGATGATTTAATCTATTTAAGTGTTGGACTGCTAGGAGAACTTGTACATTTATGATAATGCAGAATTA
  GGAAAACGGTTCACCAGTGTTTAGTTTTATATTGAGGTGCTCAGGTTGGAATAAAGTGGTATAAAAAGC
  AA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004162.5
• Summary: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).[UniProtKB/Swiss-Prot Function]
• Locus ID: 5868
• MW: 53.6