M-CSF (CSF1) (NM_000757) Human 3' UTR Clone

CAT#: SC210902

3' UTR clone of colony stimulating factor 1 (macrophage) (CSF1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: M-CSF
• Synonyms: CSF-1; MCSF
• ACCN: NM_000757
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC210902 3’UTR clone of NM_000757
  The sequence shown below is from the reference sequence of NM_000757. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GATGACAGACAGGTGGAACTGCCAGTGTAGAGGGAATTCTAAGCTGGACGCACAGAACAGTCTCTCCGT
  GGGAGGAGACATTATGGGGCGTCCACCACCACCCCTCCCTGGCCATCCTCCTGGAATGTGGTCTGCCCT
  CCACCAGAGCTCCTGCCTGCCAGGACTGGACCAGAGCAGCCAGGCTGGGGCCCCTCTGTCTCAACCCGC
  AGACCCTTGACTGAATGAGAGAGGCCAGAGGATGCTCCCCATGCTGCCACTATTTATTGTGAGCCCTGG
  AGGCTCCCATGTGCTTGAGGAAGGCTGGTGAGCCCGGCTCAGGACCCTCTTCCCTCAGGGGCTGCACCC
  TCCTCTCACTCCCTTCCATGCCGGAACCCAGGCCAGGGACCCACCGGCCTGTGGTTTGTGGGAAAGCAG
  GGTGGACGCTGAGGAGTGAAAGAACCCTGCACCCAGAGGGCCTGCCTGGTGCCAAGGTATCCCAGCCTG
  GACAGGCATGGACCTGTCTCCAGAGAGAGGAGCCTGAAGTTCGTGGGGCGGGACAGCGTCGGCCTGATT
  TCCCGTAAAGGTGTGCAGCCTGAGAGACGGGAAGAGGAGGCCTCTGGACCTGCTGGTCTGCACTGACAG
  CCTGAAGGGTCTACACCCTCGGCTCACCTAAGTGCCCTGTGCTGGTTGCCAGGCGCAGAGGGGAGGCCA
  GCCCTGCCCTCAGGACCTGCCTGACCTGCCAGTGATGCCAAGAGGGGGATCAAGCACTGGCCTCTGCCC
  CTCCTCCTTCCAGCACCTGCCAGAGCTTCTCCAGGAGGCCAAGCAGAGGCTCCCCTCATGAAGGAAGCC
  ATTGCACTGTGAACACTGTACCTGCCTGCTGAACAGCCTGCCCCCGTCCATCCATGAGCCAGCATCCGT
  CCGTCCTCCACTCTCCAGCCTCTCCCCAGCCTCCTGCACTGAGCTGGCCTCACCAGTCGACTGAGGGAG
  CCCCTCAGCCCTGACCTTCTCCTGACCTGGCCTTTGACTCCCCGGAGTGGAGTGGGGTGGGAGAACCTC
  CTGGGCCGCCAGCCAGAGCCGGTCTTTAGGCTGTGTTGTTCGCCCAGGTTTCTGCATCTTGCACTTTGA
  CATTCCCAAGAGGGAAGGGACTAGTGGGAGAGAGCAAGGGAGGGGAGGGCACAGACAGAGAGGCTACAG
  GGCGAGCTCTGACTGAAGATGGGCCTTTGAAATATAGGTATGCACCTGAGGTTGGGGGAGGGTCTGCAC
  TCCCAAACCCCAGCGCAGTGTCCTTTCCCTGCTGCCGACAGGAACCTGGGGCTGAACAGGTTATCCCTG
  TCAGGAGCCCTGGACTGGGCTGCATCTCAGCCCCACCTGCATGGTATCCAGCTCCCATCCACTTCTCAC
  CCTTCTTTCCTCCTGACCTTGGTCAGCAGTGATGACCTCCAACTCTCACCCACCCCCTCTACCATCACC
  TCTAACCAGGCAAGCCAGGGTGGGAGAGCAATCAGGAGAGCCAGGCCTCAGCTTCCAATGCCTGGAGGG
  CCTCCACTTTGTGGCCAGCCTGTGGTGGTGGCTCTGAGGCCTAGGCAACGAGCGACAGGGCTGCCAGTT
  GCCCCTGGGTTCCTTTGTGCTGCTGTGTGCCTCCTCTCCTGCCGCCCTTTGTCCTCCGCTAAGAGACCC
  TGCCCTACCTGGCCGCTGGGCCCCGTGACTTTCCCTTCCTGCCCAGGAAAGTGAGGGTCGGCTGGCCCC
  ACCTTCCCTGTCCTGATGCCGACAGCTTAGGGAAGGGCAGTGAACTTGCATATGGGGCTTAGCCTTCTA
  GTCACAGCCTCTATATTTGATGCTAGAAAACACATATTTTTAAATGGAAGAAAAATAAAAAGGCATTCC
  CCCTTCATCCCCCTACCTTAAACATATAATATTTTAAAGGTCAAAAAAGCAATCCAACCCACTGCAGAA
  GCTCTTTTTGAGCACTTGGTGGCATCAGAGCAGGAGGAGCCCCAGAGCCACCTCTGGTGTCCCCCCAG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000757.6
• Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
• Locus ID: 1435
• MW: 71.9