PLEKHM2 (NM_015164) Human 3' UTR Clone

CAT#: SC210462

3' UTR clone of pleckstrin homology domain containing family M (with RUN domain) member 2 (PLEKHM2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLEKHM2
• Synonyms: SKIP
• ACCN: NM_015164
• Insert Size: 865 bp
• Sequence Data:
  Insert Sequence

  >SC210462 3’UTR clone of NM_015164
  The sequence shown below is from the reference sequence of NM_015164. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGCCGAGCCTCCCGAGACCCCTGGTGCTGAGGCAGAGCTGGTTGGCGTCCCTGGTGGGCAGGAAAGGAA
  GGCACGCCAGCCGGCAGGCACACTGTCACGGCTGTTGTCATGCTGTCGGGAGCCTACAGTCCACCCCTG
  CCCTGGGCGGCAGAACCACCGAGTGTGGCTTAAGACAGGGTCCCTCCACTCCAGGGATCCAGATCAGGT
  GCCCGGCACCCCTGGGCATCCTGCCCGACAGGTAGCGAATGGAGGTCGCTGGGGGCAGAGGGTCCGAGC
  CCTGTGGGCTCTGCGGATGCACGCCCTCCTCCCGGGCCTCCGCCTCAGTCTGCAGAATTTCTGCCGAGT
  GGCACCGAGAACACCATCCATCTAAGGACGAACAAAAGAACCAGGAGGGCGGGACCCCCCTCTTCCTCT
  CCTGGGTTGGGGGCTGGGGCCCTGAGTGCCCAGCCATCCTTGTTCGTGTTTGAACACTCTCCTGGCCAC
  GTGGGGAAGCGGGAACACGGGGTGTCTGCGCATGTTTCCTCCTCCTAGCTCCATCACTGCGCACACAGC
  TGCCTGCCTCGCCAGATGCAGGGGGGCGGGCAGCCCTCCCTGGCTGCCAGGAGGCTCTGCATGCCCACA
  GTCCTGCCCTGCCTGTCCCCTCAACCCGGCAGTGCCTGTAGCACCGAGGAGCAAAGGGGGTGGATGGGG
  GGCTTGGAGAAGGGCGGAGCCCACCAGCCTGGCATCCATGTTGACATCTTCTGACTGTCCCCTGCTTGG
  CTGGAGCCAGGCCCTTCCCTAGAGTTTCGTCAAGAGCCTCCTGGGGAAGGGGTCAGGTGGTTTGGGTTT
  TGTTTTTTAAAATAAAATAGACATGTTATATTGCCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_015164.4
• Summary: This gene encodes a protein that binds the plus-end directed microtubule motor protein kinesin, together with the lysosomal GTPase Arl8, and is required for lysosomes to distribute away from the microtubule-organizing center. The encoded protein belongs to the multisubunit BLOC-one-related complex that regulates lysosome positioning. It binds a Salmonella effector protein called Salmonella induced filament A and is a critical host determinant in Salmonella pathogenesis. It has a domain architecture consisting of an N-terminal RPIP8, UNC-14, and NESCA (RUN) domain that binds kinesin-1 as well as the lysosomal GTPase Arl8, and a C-terminal pleckstrin homology domain that binds the Salmonella induced filament A effector protein. Naturally occurring mutations in this gene lead to abnormal localization of lysosomes, impaired autophagy flux and are associated with recessive dilated cardiomyopathy and left ventricular noncompaction. [provided by RefSeq, Feb 2017]
• Locus ID: 23207
• MW: 30.6