LAT2 (SLC7A8) Mouse Monoclonal Antibody [Clone ID: OTI5A9]

CAT#: TA500503S

Anti-SLC7A8 (LAT2) mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC7A8 (Cat# RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8(Cat# TA500503). Positive lysates LY402177 (100ug) and LC402177 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC7A8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500503)

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  Anti-SLC7A8 mouse monoclonal antibody (TA500503) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8 (RC208586).

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  Immunoprecipitation (IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC208586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC7A8 antibody (TA500503), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5A9
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:500, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SLC7A8 expression vector .
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.4 kDa
• Gene Name: solute carrier family 7 member 8
• Database Link:
  NP_036376
  Entrez Gene 50934 MouseEntrez Gene 84551 RatEntrez Gene 23428 Human
  UniProt ID Q9UHI5
• Background: Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.
• Synonyms: LAT2; LPI-PC1

Reference Data

• Protein Families: Druggable Genome, Transmembrane