Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1B12]

CAT#: TA500021AM

CK8 mouse monoclonal antibody, clone 1B12, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KRT8 (Cat# RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8 (Cat# TA500021)(1:500).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-KRT8 monoclonal antibody at 1:1000 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.

• Anti-KRT8 mouse monoclonal antibody (TA500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).

• Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (TA500021).

• Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500021).

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Specifications

Product Data

• Clone Name: OTI1B12
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2500~5000, IHC 1:50 - 1:150, IF 1:50 - 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: keratin 8
• Database Link:
  NP_002264
  Entrez Gene 16691 MouseEntrez Gene 25626 RatEntrez Gene 486513 DogEntrez Gene 3856 Human
  UniProt ID P05787
• Background: Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO

Reference Data

• Protein Families: Druggable Genome