Nck beta (NCK2) Rabbit Polyclonal Antibody

CAT#: TA354479

Rabbit anti NCK2 Polyclonal Antibody

Product Images

WB: The cell lysate derived from MCF-7 was immunoblotted by the Rabbit anti-NCK2 antibody at 1:500. An immunoreactive band is observed at~45 kDa.

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  IHC: Human breast cancer stained with Rabbit anti-NCK2 antibody at 1:50 for 30 min, RT. (Staining of formalinfixed tissues requires boiling tissue section in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the C-terminus of human NCK2 protein.
• Formulation: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: NCK adaptor protein 2
• Database Link:
  NP_001004720
  Entrez Gene 8440 Human
  UniProt ID O43639
• Background: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
• Synonyms: GRB4; NCKbeta

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway