Phosphoribosyl pyrophosphate amidotransferase (PPAT) Rabbit Polyclonal Antibody

CAT#: TA350023

Rabbit Polyclonal Anti-PPAT Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-5: Hela cells Raji cells Jurkat cells A549 cells NIH/3T3 cells Primary antibody: TA350023 (PPAT Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 20 seconds

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350023 (PPAT Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350023 (PPAT Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA350023 (PPAT Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA350023 (PPAT Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela, Raji, Jurkat, A549 and NIH/3T3 cells
  IHC: 100-300
  Positive control: Human cervical cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human PPAT
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57 kDa
• Gene Name: phosphoribosyl pyrophosphate amidotransferase
• Database Link:
  NP_002694
  Entrez Gene 117544 RatEntrez Gene 231327 MouseEntrez Gene 5471 Human
  UniProt ID Q06203
• Background: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
• Synonyms: ATASE; GPAT; PRAT

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways, Purine metabolism