p53 (TP53) Rabbit Polyclonal Antibody

CAT#: TA321153

Anti-TP53 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-4: HT29 HepG2 Hela 231 cell lysates Primary antibody: TA321153 (TP53 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 500-2000
  WB positive control: HT29, HepG2, Hela, 231 cell lysates
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 1-233 amino acids of human tumor protein p53
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44 kDa
• Gene Name: tumor protein p53
• Database Link:
  NP_000537
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: This gene encodes tumor protein p53; which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest; apoptosis; senescence; DNA repair; or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines; where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation; DNA-binding; and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion; and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site; and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies; but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms; which can regulate p53 transcriptional activity.
• Synonyms: BCC7; LFS1; P53; TRP53

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway