RANBP1 Rabbit Polyclonal Antibody

CAT#: TA308769

Rabbit Polyclonal antibody to Ran BP1 (RAN binding protein 1)

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 12% SDS PAGE. TA308769 diluted at 1:1000.

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  Sample (30 ug of whole cell lysate). A: HeLa S3. 12% SDS PAGE. TA308769 diluted at 1:1000

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ran BP-1 (TA308769) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Rat, Cow)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 201 of Ran BP1 (Uniprot ID#P43487)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: RAN binding protein 1
• Database Link:
  NP_002873
  Entrez Gene 19385 MouseEntrez Gene 360739 RatEntrez Gene 5902 Human
  UniProt ID P43487
• Background: Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. [provided by RefSeq]
• Synonyms: HTF9A
• Note: Seq homology of immunogen across species: Rat (97%), cow (88%)