Germany
Japan
United Kingdom
China
Cas9 Insertion at AAVS1
Cas9 stable human cell lines for CRISPR mediated genome editing, the best way is to insert Cas9 at a safe harbor location, AAVS1 locus. CRISPR technology can be used to achieve this. Two components are needed: All-in-one AAVS1 gRNA targeting vector and Cas9 AAVS1 donor vector. AAVS1 targeting vector cuts AAVS1 locus, Cas9 and a selection cassette from the donor vector containing the AAVS1 homologous arms will integrate into the AAVS1 locus.
AAVS1 gRNA Vector
| Cat # | Product Name | Description | Price | Availability | Manual |
|---|---|---|---|---|---|
| GE100023 | pCas-Guide-AAVS1 | AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector | $350 | In Stock |
Cas9 AAVS1 Donor Vectors
| Cat # | Product Name | Description | Price | Availability | Manual |
|---|---|---|---|---|---|
| GE100037 | pAAVS1-Cas9-Puro-DNR | Cas9 expression vector in AAVS1 donor vector, puromycin selection (10 ug) | $360 | In Stock | |
| GE100038 | Cas9 AAVS1 insertion kit (Puro) | Vector kit to insert Cas9 at AAVS1 locus (puro selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-Puro donor (cat# GE100037) and a scramble gRNA control (cat# GE100003) | $670 | 1 Week | |
| GE100039 | pAAVS1-Cas9-BSD-DNR | Cas9 expression vector in AAVS1 donor vector, blasticidin selection (10ug) | $360 | In Stock | |
| GE100040 | Cas9 AAVS1 insertion kit (BSD) | Vector kit to insert Cas9 at AAVS1 locus (blasticidin selection), including AAVS1 gRNA vector (cat# GE100023), Cas9-BSD donor (cat# GE100039), and a scramble gRNA control (cat# GE100003) | $670 | 1 Week |
Cas9 AAVS1 Insertion Diagram
