Cas9 Insertion at AAVS1

Cas9 stable human cell lines for CRISPR mediated genome editing, the best way is to insert Cas9 at a safe harbor location, AAVS1 locus. CRISPR technology can be used to achieve this. Two components are needed: All-in-one AAVS1 gRNA targeting vector and Cas9 AAVS1 donor vector. AAVS1 targeting vector cuts AAVS1 locus, Cas9 and a selection cassette from the donor vector containing the AAVS1 homologous arms will integrate into the AAVS1 locus.