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Rabbit Monoclonal Anti-Human IgE Antibody, Clone RM122
| Applications | ELISA: 10ng/well - 100ng/well for Capture;, 0.01ug/mL - 0.1ug/mL for Detection;, ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL |
| Reactivities | Human IgE |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 904, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Purified
| Applications | ELISA: 1/4,000-1/8,000. Flow Cytometry. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Human IgE goat polyclonal antibody, HRP
| Applications | ELISA: 1/10000-1/100000. |
| Reactivities | Human, Monkey |
| Conjugation | HRP |
Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Canine IgE mouse monoclonal antibody, clone E6-2A1, Purified
| Applications | Western Blot. ELISA: The antibody may be used as a Capture antibody in ELISA in combination with SM1499B as detecting antibody. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone 090-18132, Purified
| Applications | ELISA. Recommended pairs for Sandwich Immunoassay: Capture / Detection: AM00919PU-N / AM00917PU-N. AM00919PU-N / AM00918PU-N. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone NI 307, FITC
| Applications | To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. General Recommended Dilutions: Histochemical Use: 1/50-1/200. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgE (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80, depending on the method used. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
| Reactivities | Human |
| Conjugation | HRP |
Goat IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Goat |
| Conjugation | Unconjugated |
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