Antibodies

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Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, TRITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to etermine its concentration in techniques as ELISA, indirect mmunoperoxidase staining of ytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/1-1/50.
ELISA: from 1/20 upwards.
Western blot: from 1/40 upwards.
Reactivities Human
Conjugation TRITC

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC

Applications To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), TRITC

Applications To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human Kappa Light Chain (free and bound) goat polyclonal antibody, TRITC

Applications ELISA, ID, IF, IHC, IP
Reactivities Human
Conjugation TRITC

Complement C3 (C3) goat polyclonal antibody, TRITC

Applications ELISA, ID, IF, IHC, IP
Reactivities Human
Conjugation TRITC

Human IgD (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC

Human IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC

Human Lambda Light Chain (free and bound) goat polyclonal antibody, TRITC

Applications ELISA, ID, IF, IHC, IP
Reactivities Human
Conjugation TRITC

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), TRITC

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), TRITC

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA, and Immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation TRITC

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), TRITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), TRITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), TRITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50. 
ELISA: from 1/200 upwards. 
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), TRITC

Applications The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation TRITC