Human IgE (Fc specific) Goat Polyclonal Antibody

CAT#: AP21480TC-N

Human IgE (Fc specific) goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP
• Recommended Dilution: Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended working dilutions: 1/10 - 1/30.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified monoclonal IgE isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: TRITC
  State: Lyophilized purified IgG fraction
  Label: Tetramethylrhodamine Isothiocyanate
  Absorption emission: 554 nm / 573 nm
  Molar radio: TRITC/IgG: ~2.0
• Reconstitution Method: Restore by adding 1,0 ml sterile distilled water.
• Concentration: 10,0 mg/ml
• Purification: Immunoaffinity Chromatography
• Conjugation: TRITC
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies crossreacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.