Human IgA (Secretory component) Mouse Monoclonal Antibody [Clone ID: NI 194-4 (A89-039)]

CAT#: AM20261TC-N

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), TRITC

Specifications

Product Data

• Clone Name: NI 194-4 (A89-039)
• Applications: ELISA, IF, IHC, IP, WB
• Recommended Dilution: To identify the presence of secretory component, free or bound, in human milk, other body fluids, cell and tissue substrates and to determine its concentration in immunofluorescence staining techniques.
  General Recommended Dilutions:
  Histochemical Use: 1/10-1/100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Highly purified Secretory component isolated from Human milk.
• Specificity: The antiserum reacts with Human secretory component, free and bound. It does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
  The reactivity of the antiserum is restricted to Secretory component as tested in Indirect Binding Immunoblotting and Indirect Immunoperoxidase staining.
• Formulation: PBS, pH 7.2 without preservatives.
  No foreign protein added.
  Label: TRITC
  State: Lyophilized purified IgG fraction.
  Label: Tetramethylrhodamine Isothiocyanate
  Absorption emission: 554 nm / 573 nm
  Molar radio: Fluorochrome/IgG: ~ 1.8
• Reconstitution Method: Restore by adding 0.5 ml sterile distilled water.
  Dilutions may be prepared by adding PBS, pH 7.2
• Concentration: 0.4 mg/ml
• Conjugation: TRITC
• Storage: Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated thawing and freezing.
• Note: Fluorescent Marker: Tetramethylrhodamine isothiocyanate isomer R with an Orange-Red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, TRITC conjugates have the advantage of significantly less photo bleaching.
  Conjugation procedure: Conjugation is carried out using a proprietary technique for the binding of TRITC, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.