Antibodies

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GFAP chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Recombinant Human GFAP (expressed in bacteria).
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.

Chicken Polyclonal Albumin Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin. The immunogen is located within amino acids 340 - 390 of Albumin.

chicken Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

Applications IF, WB
Reactivities Rat
Conjugation Unconjugated
Immunogen Recombinant and purified bovine GFAP

Chicken Polyclonal Anti-Beta-actin Antibody

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human beta-actin.

Chicken polyclonal anti-TUBA1A(alpha Tubulin) antibody, Loading control

Applications WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.

lacZ chicken polyclonal antibody, Purified

Applications IF, IHC, WB
Conjugation Unconjugated
Immunogen Purified Beta-Galactosidase (LacZ from E.coli) protein emulsified in Freund's adjuvants.
After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using Beta-Galactosidase conjugated to an Agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 mg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Nes chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Mouse, Rat
Conjugation Unconjugated
Immunogen Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence.
Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

GAD67 (GAD1) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products.
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column.

Chicken Polyclonal Anti-GAPDH Antibody

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH. The immunogen is located within amino acids 230 - 280 of GAPDH.

Chicken Anti-Vimentin Antibody

Applications IF, WB
Reactivities Rat
Conjugation Unconjugated
Immunogen Recombinant human vimentin purified from E. coli

L1 / ORF2 chicken polyclonal antibody, Ig Fraction

Applications ELISA, WB
Reactivities Chimpanzee, Human
Conjugation Unconjugated
Immunogen Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein

6xHistidine Epitope Tag (HHHHHH) chicken polyclonal antibody, Purified

Applications IP, WB
Conjugation Unconjugated
Immunogen 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants.
After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix.
To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.

Chicken Polyclonal GAPDH Antibody

Applications WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen GAPDH antibody was raised against a 16 amino acid peptide from near the amino terminus of human GAPDH.

Actin chicken polyclonal antibody, Aff - Purified

Applications WB
Reactivities Rabbit
Conjugation Unconjugated
Immunogen Chickens were immunized with actin purified from Rabbit muscle.
After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.