MED6 Human shRNA Lentiviral Particle (Locus ID 10001)

CAT#: TL311517V

MED6 - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Product Images

GFP signal was observed under microscope at 48 hours after transduction of TL311517A virus into HEK293 cells. TL311517A virus was prepared using lenti-shRNA TL311517A and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of TL311517B virus into HEK293 cells. TL311517B virus was prepared using lenti-shRNA TL311517B and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL311517C] virus into HEK293 cells. [TL311517C] virus was prepared using lenti-shRNA [TL311517C] and TR30037 packaging kit.

• 

  GFP signal was observed under microscope at 48 hours after transduction of [TL311517D] virus into HEK293 cells. [TL311517D] virus was prepared using lenti-shRNA [TL311517D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 10001
• Synonyms: ARC33; NY-REN-28
• Vector: pGFP-C-shLenti
• Format: Lentiviral particles
• RefSeq: NM_001284209, NM_001284210, NM_001284211, NM_005466, NM_005466.1, NM_005466.2, NM_005466.3, NM_001284210.1, NM_001284209.1, NM_001284211.1, BC004106, BC004106.2, BM152825, BM802752, BM999935, NM_005466.4
• UniProt ID: O75586
• Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).