Mir122 Mouse MicroRNA Expression Plasmid (MI0000256)

CAT#: SC400744

Expression plasmid for mouse microRNA Mir122

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AGCUGUGGAGUGUGACAAUGGUGUUUGUGUCCAAACCAUCAAACGCCAUUAUCACACUAAAUAGCU
  Mature Sequence:

  UGGAGUGUGACAAUGGUGUUUG
  Full insert

  >MI0000256 AACCATGACGAGGTGAGAGTGGGTGGGATCTATCACCTAATGGAGTTATTTCCTTGTATGATGTGGTTTG
  TAAGAAGTGTCTGCCTCTTCCTGGAATTCAAGCCTTTTAGGAAACAGATGTAAACAAATTTCCATCCTGG
  TAAAGGCCTTTGTGAGCCCTCCTTGTTATTCAAGATCCCGGGGCTCTTCCCTATGCTCCAGTTTCAGCGT
  TTGGAACCACTGAGGAGTCTGTGACAGACAACGGAGGAATGTGAAGGGAAGGTTAATACCCTTGTGCCTG
  ACAGACTTTCCTTAGCAGAGCTGTGGAGTGTGACAATGGTGTTTGTGTCCAAACCATCAAACGCCATTAT
  CACACTAAATAGCTACTGCTAGGCAATCCGTCCACTCCACGCGTGACTTGACGTCTGTTCTCTCCGAGCA
  AGAAGTTCTGTCTTGTGCTAGAGTTCTCCATTGTGTGTAATGTATGAATAAAATCTGGTCCTCTTGTGCT
  TATAACTGTACATCTGACTGACGCCTTCCCCTCGTGTGCTATTGTGAAATGAATAATGCTATAAAGGAAA
  AGACCAGTAAAATCCAACCTCTCCTATGGGACCCTCCCAACACTGACTACCCTGACACCCAATTTATTTC
  CAGCACTAGGCCCACTCTCGATTTTGCTCTGA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000256
• Synonyms: Mirn122a mmu-mir-122
• Locus ID: 387231