CD33 (NM_001772) Human Recombinant Protein

CAT#: TP762715

Purified recombinant protein of Human CD33 molecule (CD33), transcript variant 1, 50Tyr-259His, with N-terminal His tag, expressed in E.coli, 50ug

Product Images

Coomassie blue staining of purified CD33 protein (Cat #TP762715). The protein was produced from E.coli.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (50Tyr-259His) of CD33
• Tag: N-His
• Predicted MW: 25.3 kDa
• Concentration: >0.05 ug/ul as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25mM Tris, 150mM NaCl, 10% glycerol, pH8.0, 1% SKL
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001763
• Locus ID: 945
• UniProt ID: P20138, Q546G0
• Cytogenetics: 19q13.41
• Refseq Size: 1466
• Refseq ORF: 1092
• Synonyms: p67; SIGLEC-3; SIGLEC3
• Summary: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).[UniProtKB/Swiss-Prot Function]
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage