Chk1 (CHEK1) (NM_001274) Human Recombinant Protein

CAT#: TP762547

Purified recombinant protein of Human CHK1 checkpoint homolog (S.pombe) (CHEK1), transcript variant 1, 50ug

Product Images

Coomassie blue staining of purified CHEK1 protein (Cat #TP762547). The protein was produced from E.coli.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region full length of CHEK1
• Tag: N-GST and C-HIS
• Predicted MW: 54.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50mM Tris, pH8.0, 8M Urea
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001265
• Locus ID: 1111
• UniProt ID: O14757
• Cytogenetics: 11q24.2
• Refseq Size: 3517
• Refseq ORF: 1428
• Synonyms: CHK1
• Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway