PGF (19-170, His-Tag) Human Recombinant Protein

CAT#: TP728135

Recombinant human PIGF-2 protein

Product Images

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Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR
• Tag: His-Tag
• Predicted MW: 18.1kDa (158aa)
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
• Purity: > 90% by SDS - PAGE
• Buffer: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Bioactivity: Measured by its binding ability in a functional ELISA with Human VEGFR1/Flt-1 (CAT# ATGP4101).
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Reference Data

• RefSeq: NP_002623.2
• Summary: PIGF-2, as know as placenta growth factor, is a member of the VEGF sub-family that a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. It is ultimately associated with angiogenesis. Specifically, it plays a role in trophoblast growth and differentiation. Also it is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure. Under normal physiologic conditions, it is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle. Recombinant human PIGF-2, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.