IBSP (17-317, His-Tag) Human Recombinant Protein

CAT#: TP724591

Recombinant human IBSP/Sialoprotein II protein

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  FSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDS SEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKA GDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESV TGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVYRT TSPPFGKTTT VEYEGEYEYT GANEYDNGYE IYESENGEPR GDNYRAYEDE YSYFKGQGYD GYDGQNYYHH Q
• Tag: His-Tag
• Predicted MW: 34.3kDa (307aa)
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
• Purity: > 90% by SDS-PAGE
• Buffer: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When cells are added to Human IBSP coated plates 3 ug/ml. This effect is more to 40%.
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Reference Data

• RefSeq: NP_004958.2
• Summary: IBSP, also known as Sialoprotein II, is a monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is synthesized by platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts and bone marrow stromal cells. It is a key mediator of the hypertrophic chondrocytes-induced angiogenesis. It was associated with the severity of histological cartilage lesions and with vascular density at the osteochondral junction. Recombinant human IBSP/Sialoprotein II, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.