NGAL (Lcn2) (NM_008491) Mouse Recombinant Protein

CAT#: TP723846

Purified recombinant protein of Mouse lipocalin 2 (Lcn2 / NGAL)

Specifications

Product Data

• Species: Mouse
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Mouse NGAL, the region of Gln21-Asn200, from gene Accession# NP_032517
• Tag: C-His
• Predicted MW: 23 kDa
• Concentration: lot specific
• Purity: >95%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 1 x PBS
• Bioactivity: The BC50 = 0.7 µM, determined by dose dependent binding of the protein (4 µM) with [Fe3+(DHBA)3] complex.
• Endotoxin: Less than 0.01 ng per µg protein as determine by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_032517
• Locus ID: 16819
• UniProt ID: P11672
• Cytogenetics: 2 22.09 cM
• Refseq Size: 853
• Refseq ORF: 600
• Synonyms: 24p3; AW212229; NRL; Sip24
• Summary: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed:12453413). Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed:15531878, PubMed:16446425). Can also bind siderophores from M.tuberculosis (By similarity).[UniProtKB/Swiss-Prot Function]