L1CAM Human Recombinant Protein

CAT#: TP721331

Biotinylated Human CD171/L1CAM Protein (C-His-Avi)

Product Images

Biotinylated Human CD171 Protein (C-His-Avi) on SDS-PAGE under reducing condition P(+) and non-reducing condition P(-). The purity of this protein appears to be greater than 90% based on Coomassie-blue staining.

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Ile20-Glu1120
• Tag: C-His-Avi
• Predicted MW: The protein has a predicted molecular weight of 127 kDa and migrates at approximately 160-180 kDa on SDS-PAGE with DTT-reduced conditions.
• Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Conjugation: Biotin
• Presentation: Purified
• Buffer: 1xPBS buffer, pH7.4
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: ELISA
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months

Reference Data

• RefSeq: NP_000416.1
• Locus ID: 3897
• UniProt ID: P32004