NKIRAS2 (NM_001144927) Human Mass Spec Standard

CAT#: PH326742

NKIRAS2 MS Standard C13 and N15-labeled recombinant protein (NP_001138399)

Product Images

Coomassie blue staining of purified NKIRAS2 protein (Cat# TP326742). The protein was produced from HEK293T cells transfected with NKIRAS2 cDNA clone (Cat# RC226742) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC226742
• Predicted MW: 21.5 kDa
• Protein Sequence:
  Sequence Link

  >RC226742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKSCKVVVCGQASVGKTSILEQLLYGNHVVGSEMIETQEDIYVGSIETDRGVREQVRFYDTRGLRDGAE
  LPRHCFSCTDGYVLVYSTDSRESFQRVELLKKEIDKSKDKKEVTIVVLGNKCDLQEQRRVDPDVAQHWAK
  SEKVKLWEVSVADRRSLLEPFVYLASKMTQPQSKSAFPLSRKNKGSGSLDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001138399
• RefSeq Size: 2493
• RefSeq ORF: 573
• Synonyms: kappaB-Ras2; KBRAS2
• Locus ID: 28511
• UniProt ID: Q9NYR9, A0A024R1Z4
• Cytogenetics: 17q21.2
• Summary: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB (By similarity).[UniProtKB/Swiss-Prot Function]