Phosphoserine phosphatase (PSPH) (NM_004577) Human Mass Spec Standard

CAT#: PH309090

PSPH MS Standard C13 and N15-labeled recombinant protein (NP_004568)

Product Images

Coomassie blue staining of purified PSPH protein (Cat# TP309090). The protein was produced from HEK293T cells transfected with PSPH cDNA clone (Cat# RC209090) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209090
• Predicted MW: 25 kDa
• Protein Sequence:
  Sequence Link

  >RC209090 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSHSELRKLFYSADAVCFDVDSTVIREEGIDELAKICGVEDAVSEMTRRAMGGAVPFKAALTERLALIQ
  PSREQVQRLIAEQPPHLTPGIRELVSRLQERNVQVFLISGGFRSIVEHVASKLNIPATNVFANRLKFYFN
  GEYAGFDETQPTAESGGKGKVIKLLKEKFHFKKIIMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAK
  WYITDFVELLGELEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004568
• RefSeq Size: 2142
• RefSeq ORF: 675
• Synonyms: PSP; PSPHD
• Locus ID: 5723
• UniProt ID: P78330, A0A024RDL3
• Cytogenetics: 7p11.2
• Summary: The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways