Chrna7 Mouse Gene Knockout Kit (CRISPR)

CAT#: KN303310LP

Chrna7 - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Chrna7
• Locus ID: 11441
• Components:

  KN303310G1, Chrna7 gRNA vector 1 in pCas-Guide CRISPR vector

  KN303310G2, Chrna7 gRNA vector 2 in pCas-Guide CRISPR vector

  KN303310LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_007390
• UniProt ID: P49582
• Synonyms: Acra7; alpha7; nAchR
• Summary: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.[UniProtKB/Swiss-Prot Function]