PAPP A (PAPPA) Human Gene Knockout Kit (CRISPR)

CAT#: KN209767

PAPPA - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: PAPP A
• Locus ID: 5069
• Components:

  KN209767G1, PAPP A gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGCTGGCCGAGCGTCCCCGC

  KN209767G2, PAPP A gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGGCTGCTGAGCGCCGCGC

  KN209767D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  ATTAGTCATG ATCCCCGCAG TTTTAACAGG GACTCATTCA ATTGGGAAGG TGGAGCGCTG GGGAGCAGAT TAGCATACGC TTGTTTACTC ATCTTCTGAG GGATTTTTTC CCCCTCTTTC CTTTCATTTT GAGAAGAAGG AGGGAGGGGA GGGGGGACTT GGGGGGGGAG AAGGGGGCTG TGGCTTGTGT TATAAAGGAC GCAAAAAATA AATAAATTAG AGCATCTTTT GGGGGGAGGG AATTCAGCGG ATCAGTCTTA AGAGGAGCTT TTTTTTGGAG CGAGAAATCA TATAAAATAA AATGAAATAA AACAAGGAGG AAGGCAACCA GCTGTTAGGG GAAAAATAAG GCAGATAAAG GAGCGGGGAG AGAAATTAAT TGCCAACCAG GAGGAGTTGG GCTGTATTTT TCAAAGGTGG GGAGAGTGGA GCACACACCT TGAGGAGGAA AGCGAGAAAG AAAAGAAAAA AGCAAGTGGA AAGGGGGGCT CGCCCAAGAA GGGTGAAGAA GCGAAGAAAG TCGAGGCGCC GAGGCTCCCA AAGCTGGCAG CTCCGGGTGG CGGTGCAGGG GCGAAGGGGG GGCGGGGGGA ACCGTCGGAC CCGGCCGACC CCCGCGCCCC GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGTAGG TGAGGGCGCC TCGGCGGGCG CTGCACCGTC CCTGCGGCCC CAGAGGCTCG CGGGTGTCTG GGCGCGGGTG GCGGGCGGGT CGGGGGCTTG CGGGCGTGTC TGTGCGAGAG CTGCCCCGCG AGCGGCGCAG AGACATCCGG GCGAGCTGAG AGCCTCACTT TGCTCCATCG GTGGAATGGG CACACTCGGA AGGCGTAGCT GCTCCATCCC TGGGAGGAAC CTGGGGAGCC CTCCTGGCGG CCCCGCGGAC CCTCGGCCAG TGAGGGCTGG TTCCCGGAGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002581
• UniProt ID: Q13219
• Synonyms: ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPP-A; PAPPA1
• Summary: This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [provided by RefSeq, Aug 2017]