Heme Oxygenase 1 (HMOX1) Human Gene Knockout Kit (CRISPR)

CAT#: KN200463LP

HMOX1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Heme Oxygenase 1
• Locus ID: 3162
• Components:

  KN200463G1, Heme Oxygenase 1 gRNA vector 1 in pCas-Guide CRISPR vector

  KN200463G2, Heme Oxygenase 1 gRNA vector 2 in pCas-Guide CRISPR vector

  KN200463LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_002133
• UniProt ID: P09601
• Synonyms: bK286B10; HMOX1D; HO-1; HSP32
• Summary: Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]